Entering edit mode
Yanyan Li
▴
20
@yanyan-li-1955
Last seen 11.2 years ago
Hi Bioconductors:
I am doing Random Block Design for single-color arrays, consider the
following target frame:
FileName Exp Trt
1 1 A
2 1 B
3 1 C
4 1 D
5 2 A
6 2 B
7 2 C
8 2 D
9 3 A
10 3 B
11 3 C
12 3 D
Each mRNA has 3 replicates (same y location and different x location),
I am
not a biology person, I hope I could express clearly.
Now I want to compare pairwise groups across the 3 experiments, treat
Exp
as block factor (random) and each mRNA is repeated.
How to incorporate random and repeated effects to lmFit command and
also
contrast.
Is anyone deal with similar situation or any other suggestions?
I appreciate all your help.
Thanks a lot
yanyan
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Yanyan Li phone: 215-503-9876, fax: 215-503-3804
MS. Biostatistician
Division of Biostatistics
Department of Pharmacology and Experimental Therapeutics
Thomas Jefferson University
1015 Chestnut St., Suite M100, Mezzanine Floor
Philadelphia, PA 19107
