Limma: setting up printer information for agilent files
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Daniel Brewer ★ 1.9k
@daniel-brewer-1791
Last seen 9.8 years ago
I am having trouble setting up the printer information for an agilent data set. These are the commands I am following: > targets <- readTargets() > stuff <- read.maimages(targets$FileName,source="agilent") > #Setup layout > stuff$genes$Block <- 1 > names(stuff$genes)[2] <- "Column" > stuff$printer <- getLayout(stuff$genes) > imageplot(log2(stuff$Rb[,1]), stuff$printer, low="white", high="red") > Error in imageplot(log2(stuff$Rb[, 1]), stuff$printer, low = "white", : > Number of image spots does not agree with layout dimensions I added Block information (there is only one block on agilent arrays) and changed a column name from "Col" to "Column". The block is 119x156. Any idea what I am doing wrong? The printer object gives this: > stuff$printer > $ngrid.r > [1] 1 > > $ngrid.c > [1] 1 > > $nspot.r > [1] 119 > > $nspot.c > [1] 156 > > attr(,"class") > [1] "PrintLayout" Thanks -- ************************************************************** Daniel Brewer, Ph.D. Email: daniel.brewer at icr.ac.uk
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