Affymetrix data analysis
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@tristan-coram-2024
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@james-w-macdonald-5106
Last seen 1 day ago
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Hi Tristan, Tristan Coram wrote: > Hi, > > > > I am trying to read in my Affymetrix CEL files (48 files, total ~600 MB) but > I keep getting memory errors. Can somebody please help me with this. Or is > therea remote server I can send my data to for computation? You don't say anything about your OS, version of R, sessionInfo(), amount of RAM, etc, etc, so really there is very little anybody can say for sure (this is like calling a mechanic on the phone and saying 'My car won't run. Can you help me?'). Some general prescriptives: 1.) Get more RAM. It's cheap these days. 2.) Use justRMA() or justGCRMA(). 3.) Use a 64-bit OS with lots of RAM. 4.) If on windows or linux, you can use RMAexpress (google to find). HTH, Jim > > > > Any help is much appreciated. > > > > Thanks > > > > > > Dr. Tristan Coram > > Postdoctoral Research Associate > > Research Plant Pathologist/Geneticist > > > > United States Department of Agriculture > > Agricultural Research Service > > Wheat Genetics, Quality Physiology & Disease Research > > > > 209 Johnson Hall > > Washington State University > > Pullman, WA 99163 > > > > Office: +1 509 335-1596 Fax: +1 509 335-2553 > > Email: tcoram at mail.wsu.edu > > > > > > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician Affymetrix and cDNA Microarray Core University of Michigan Cancer Center 1500 E. Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623 ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues.
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and even more importantly, please read the posting guide before posting - it is *important* James W. MacDonald wrote: > Hi Tristan, > > Tristan Coram wrote: >> Hi, >> >> >> >> I am trying to read in my Affymetrix CEL files (48 files, total ~600 MB) but >> I keep getting memory errors. Can somebody please help me with this. Or is >> therea remote server I can send my data to for computation? > > You don't say anything about your OS, version of R, sessionInfo(), > amount of RAM, etc, etc, so really there is very little anybody can say > for sure (this is like calling a mechanic on the phone and saying 'My > car won't run. Can you help me?'). > > Some general prescriptives: > > 1.) Get more RAM. It's cheap these days. > 2.) Use justRMA() or justGCRMA(). > 3.) Use a 64-bit OS with lots of RAM. > 4.) If on windows or linux, you can use RMAexpress (google to find). > > HTH, > > Jim > > >> >> >> Any help is much appreciated. >> >> >> >> Thanks >> >> >> >> >> >> Dr. Tristan Coram >> >> Postdoctoral Research Associate >> >> Research Plant Pathologist/Geneticist >> >> >> >> United States Department of Agriculture >> >> Agricultural Research Service >> >> Wheat Genetics, Quality Physiology & Disease Research >> >> >> >> 209 Johnson Hall >> >> Washington State University >> >> Pullman, WA 99163 >> >> >> >> Office: +1 509 335-1596 Fax: +1 509 335-2553 >> >> Email: tcoram at mail.wsu.edu >> >> >> >> >> >> >> [[alternative HTML version deleted]] >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at stat.math.ethz.ch >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > > -- Robert Gentleman, PhD Program in Computational Biology Division of Public Health Sciences Fred Hutchinson Cancer Research Center 1100 Fairview Ave. N, M2-B876 PO Box 19024 Seattle, Washington 98109-1024 206-667-7700 rgentlem at fhcrc.org
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