expression values
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Jingqin luo ▴ 30
@jingqin-luo-320
Last seen 10.3 years ago
Hi,there, Recently, I used the 3 functions expresso, rma and justRMA to compute the expression for a same chip. The 3 outputs are not the same, kind of shifting above a little bit. Shouldn't they be same or is there a way to keep them the same? Thanks and have a good day!
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Ben Bolstad ★ 1.1k
@ben-bolstad-93
Last seen 10.3 years ago
This should be fixed in the 1.2 release. You should be getting the same values from each of three methods. Ben On Thu, 2003-06-05 at 09:50, Jingqin luo wrote: > Hi,there, > Recently, I used the 3 functions expresso, rma and > justRMA to compute the expression for a same chip. The > 3 outputs are not the same, kind of shifting above a > little bit. Shouldn't they be same or is there a way > to keep them the same? > Thanks and have a good day! > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor -- Ben Bolstad <bolstad@stat.berkeley.edu>
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After I installed the new version of R and bioconductor , the expression values obtained from the three functions(expresso,rma and justRMA) are still different. Am I wrong in setting some of the auguments? Here are the functions I used: (1)affy3chip1 <- ReadAffy ( filenames = c ( " 1674.cel" ,"1675.cel")) try.rma<-expresso(affy3chip1,bgcorrect.method="rma",normalize.method=" quantiles",pmcorrect.method="pmonly",summary.method="medianpolish") (2)try.rma1 <- rma( affy3chip1, verbose=TRUE, destructive=TRUE,normalize=TRUE,background=TRUE,bgversion=2) (3)try.rma2 <- justRMA ( filenames = c("1674.cel","1675.cel"),normalize=TRUE,background=TRUE,bgversion=1,de structive=TRUE) BTW,besides justRMA, is ther any other function used for calculation of expression values and at the same time avoid exhausting the memory? --- Ben Bolstad <bolstad@stat.berkeley.edu> wrote: > This should be fixed in the 1.2 release. You should > be getting the same > values from each of three methods. > > Ben > > > > > On Thu, 2003-06-05 at 09:50, Jingqin luo wrote: > > Hi,there, > > Recently, I used the 3 functions expresso, rma > and > > justRMA to compute the expression for a same chip. > The > > 3 outputs are not the same, kind of shifting above > a > > little bit. Shouldn't they be same or is there a > way > > to keep them the same? > > Thanks and have a good day! > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor@stat.math.ethz.ch > > > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > -- > Ben Bolstad <bolstad@stat.berkeley.edu> >
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On Sun, 8 Jun 2003, Jingqin luo wrote: SNIP > > BTW,besides justRMA, is ther any other function used > for calculation of expression values and at the same > time avoid exhausting the memory? > > > all justRMA does is avoid saving MMs. so data is at least 1/2 as big. for PM-only measures you can easily rewrite justRMA (contributed by James MacDonald) to create, e.g. justpmonlyliwong. look though the code of justRMA. all you have to do is change the calls to background subtraction, normalization, etc... if you write something for other measures, contributions are welcomed. for measures that use MM, itll be a bit harder cuase now there are twice as much data. > --- Ben Bolstad <bolstad@stat.berkeley.edu> wrote: > > This should be fixed in the 1.2 release. You should > > be getting the same > > values from each of three methods. > > > > Ben > > > > > > > > > > On Thu, 2003-06-05 at 09:50, Jingqin luo wrote: > > > Hi,there, > > > Recently, I used the 3 functions expresso, rma > > and > > > justRMA to compute the expression for a same chip. > > The > > > 3 outputs are not the same, kind of shifting above > > a > > > little bit. Shouldn't they be same or is there a > > way > > > to keep them the same? > > > Thanks and have a good day! > > > > > > _______________________________________________ > > > Bioconductor mailing list > > > Bioconductor@stat.math.ethz.ch > > > > > > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > > -- > > Ben Bolstad <bolstad@stat.berkeley.edu> > > > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor >
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