limma: get list of genes after classifyTestsF
1
0
Entering edit mode
@bandyopadhyay-somnath-som-1930
Last seen 10.3 years ago

I am using limma to analyze affy data. i made some contrasts and using classifyTestsF(object,p.value=0.01) I get the following results:

  E.OvsC.O I.OvsC.O IM.OvsC.O   E.TvsC.T I.TvsC.T
 IM.TvsC.T C.TvsC.O  E.TvsE.O IM.TvsIM.O M.TvsM.O
-1      34     229     798     369     469     430      26     146    1938     820
 0   54582   54142   53302   54105   53955   53971   54611   54417   50907   53367
 1      59     304     575     201     251     274      38     112    1830     488

Now I wanna make a list of the probests which change in every contrast (increases and decreases separately). how do I make a list of these probesets. Any help would be appreciated and thanks in advance.
Som.

limma classifyTestsF • 948 views
ADD COMMENT
1
Entering edit mode
@gordon-smyth
Last seen 2 minutes ago
WEHI, Melbourne, Australia

The output you show is from summary and decideTests:

results <- classifyTestsF(object,p.value=0.01)
summary(decideTests(results))

Suppose you want to see genes that are significantly up for every contrast. The following code will give you a topTable of just those genes:

i <- apply(results>0, 1, all)
topTable(fit[i,], n=Inf)

To see those that are significantly down for every contrast:

i <- apply(results<0, 1, all)
topTable(fit[i,], n=Inf)
ADD COMMENT

Login before adding your answer.

Traffic: 1362 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6