Dear list, I am wondering if I have any other normalization options besides justRMA() and justGCRMA() if I want to normalize 60 U133 Plus 2.0 arrays on a machine with 2 GB of RAM. Since I run out of memory when using ReadAffy on as few as 15 arrays, I suppose there really is no way to use MBEI, for example, correct? Thanks. Kamila

Hi Kamila, Currently you are limited by the amount of RAM you have. Technically you _could_ write a just.li.wong() that bypasses the explicit creation of an AffyBatch (as do justRMA() and justGCRMA()), and quite possibly you would be able to get away with just 2Gb RAM. Best, Jim -- James W. MacDonald, M.S. Biostatistician Affymetrix and cDNA Microarray Core University of Michigan Cancer Center 1500 E. Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623 >>> On 3/6/2007 at 12:32 PM, <knaxerov at="" ix.urz.uni-heidelberg.de=""> wrote: > Dear list, > > I am wondering if I have any other normalization options besides > justRMA() and justGCRMA() if I want to normalize 60 U133 Plus 2.0 > arrays on a machine with 2 GB of RAM. Since I run out of memory when > using ReadAffy on as few as 15 arrays, I suppose there really is no > way to use MBEI, for example, correct? > > Thanks. > Kamila > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues.