Hi, When running lumi, I met some problems. Here is the workflow: >library(lumi) Loading required package: Biobase Loading required package: tools Welcome to Bioconductor Vignettes contain introductory material. To view, type 'openVignette()'. To cite Bioconductor, see 'citation("Biobase")' and for packages 'citation(pkgname)'. Loading required package: affy Loading required package: affyio Loading required package: mgcv This is mgcv 1.3-23 Loading required package: annotate >MyFileName <- 'in.txt' >MyProject.lumi <- lumiR(MyFileName, lib='lumiHumanV1') Loading required package: lumiHumanV1 Warning message: Duplicated IDs found and were merged! in: addNuId2lumi(x.lumi, lib = lib) >load(MyProject.lumi) Error in load(MyProject.lumi) : bad 'file' argument >data(MyProject.lumi) Warning message: data set 'MyProject.lumi' not found in: data(MyProject.lumi) There are 47293 targetIDs with 17 columns in the BeadStudio output text file: TargetID MIN_Signal-1523532052_A AVG_Signal-1523532052_A MAX_Signal-1523532052_A NARRAYS-1523532052_A ARRAY_STDEV- 1523532052_A BEAD_STDEV-1523532052_A Avg_NBEADS-1523532052_A Detection-1523532052_A MIN_Signal-1523532052_C AVG_Signal- 1523532052_C MAX_Signal-1523532052_C NARRAYS-1523532052_C ARRAY_STDEV-1523532052_C BEAD_STDEV-1523532052_C Avg_NBEADS- 1523532052_C Detection-1523532052_C No Duplicated IDs were found before loading the data. Any help will be appreciated. Longsi Ran M.D. _________________________________________________________________ your stats fix instantly http://sports.sympatico.msn.ca/NHL/NHL_Stats_Agent

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