Why don't you use the Gene Annotation tool in EzArray? You don't even need to write any codes. Try it at [1]http://xulab.wisc.edu/ezarray/ (only guest account is allowed). Ron Marc Carlson wrote: Pengcheng Lu wrote: Greetings- I want to annotate the gene symbols and other gene information for affy chip hgu133a2. Tried many times but failed. Can you tell me how I can finish it using limma or other packages in R? Thanks, Pengcheng Lu [[alternative HTML version deleted]] _______________________________________________ Bioconductor mailing list [2]Bioconductor at stat.math.ethz.ch [3]https://stat.ethz.ch/mailman/listinfo/bioconductor Search the archives: [4]http://news.gmane.org/gmane.science.biology.informatics .conductor Have you tried using the annotation package yet? [5]http://bioconductor.org/packages/2.1/data/annotation/html/hgu133a2. db.html Here is another link to give you some directions about how to use annotation packages in case you are new to this: [6]http://bioconductor.org/packages/bioc/vignettes/annotate/inst/doc/a nnotate.p df But if you have already tried these things, can you please tell me what you have tried so far ( with results to a sessionInfo() in R) ? We even have a posting guide to help you know what sorts of things to put into your post here: [7]http://www.bioconductor.org/docs/postingGuide.html Maybe if I know a little bit more about what you are trying to do I can help you out better. ;) Marc _______________________________________________ Bioconductor mailing list [8]Bioconductor at stat.math.ethz.ch [9]https://stat.ethz.ch/mailman/listinfo/bioconductor Search the archives: [10]http://news.gmane.org/gmane.science.biology.informatic s.conductor References 1. http://xulab.wisc.edu/ezarray/ 2. mailto:Bioconductor at stat.math.ethz.ch 3. https://stat.ethz.ch/mailman/listinfo/bioconductor 4. http://news.gmane.org/gmane.science.biology.informatics.conductor 5. http://bioconductor.org/packages/2.1/data/annotation/html/hgu133 a2.db.html 6. http://bioconductor.org/packages/bioc/vignettes/annotate/inst/do c/annotate.pdf 7. http://www.bioconductor.org/docs/postingGuide.html 8. mailto:Bioconductor at stat.math.ethz.ch 9. https://stat.ethz.ch/mailman/listinfo/bioconductor 10. http://news.gmane.org/gmane.science.biology.informatics.conductor