Working with Affymetrix Files
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A A ▴ 30
@a-a-2865
Last seen 10.3 years ago
Hello Everyone, I am very new to using R and Bioconductor, so please bare with me. I have Affymetrix files that are in CEL format and i am trying to convert them into text files so that I can work with them. I have been trying to use the command justSNPRMA, but I am not sure what to do after I run the command. I tried searching on the net to see if there were step-by-step instructions for doing this, but I have not been successful. Would someone direct me to such a site or explain this process to me? Thanks, AA _________________________________________________________________ [[elided Hotmail spam]] ntrosrchcashback [[alternative HTML version deleted]]
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@james-w-macdonald-5106
Last seen 6 minutes ago
United States
Hi AA, A A wrote: > Hello Everyone, > > I am very new to using R and Bioconductor, so please bare with me. I > have Affymetrix files that are in CEL format and i am trying to > convert them into text files so that I can work with them. I have > been trying to use the command justSNPRMA, but I am not sure what to > do after I run the command. I tried searching on the net to see if > there were step-by-step instructions for doing this, but I have not > been successful. Would someone direct me to such a site or explain > this process to me? You might want to look at the posting guide, as this message fails to meet even the minimum requirements. Some questions for you: 1.) What kind of Affymetrix files? 2.) What does 'work with them' mean? 3.) What (exact) code have you tried? 4.) What is your output from sessionInfo()? 5.) Who are you (anonymous questions are less likely to be answered)? Best, Jim > > Thanks, > > AA > > _________________________________________________________________ > [[elided Hotmail spam]] > > ntrosrchcashback [[alternative HTML version deleted]] > > _______________________________________________ Bioconductor mailing > list Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor Search the > archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician Affymetrix and cDNA Microarray Core University of Michigan Cancer Center 1500 E. Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623
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You might want to try *CEL File Conversion* Tool found at http://www.affymetrix.com/support/developer/tools/devnettools.affx. This is used to . .. "convert all Affymetrix CEL files in a specified directory from version 3 format (ASCII - MAS 5 compatible format) _to and from_ version 4 format (binary - GCOS compatible format). You files may be version 4. If you convert them to version 3, you may have what you want. It is also possible to convert back to version 4 after some processing. Matthew McCormack James W. MacDonald wrote: > Hi AA, > > A A wrote: >> Hello Everyone, >> >> I am very new to using R and Bioconductor, so please bare with me. I >> have Affymetrix files that are in CEL format and i am trying to >> convert them into text files so that I can work with them. I have >> been trying to use the command justSNPRMA, but I am not sure what to >> do after I run the command. I tried searching on the net to see if >> there were step-by-step instructions for doing this, but I have not >> been successful. Would someone direct me to such a site or explain >> this process to me? > > You might want to look at the posting guide, as this message fails to > meet even the minimum requirements. Some questions for you: > > 1.) What kind of Affymetrix files? > > 2.) What does 'work with them' mean? > > 3.) What (exact) code have you tried? > > 4.) What is your output from sessionInfo()? > > 5.) Who are you (anonymous questions are less likely to be answered)? > > Best, > > Jim > > >> >> Thanks, >> >> AA >> >> _________________________________________________________________ >> [[elided Hotmail spam]] >> >> ntrosrchcashback [[alternative HTML version deleted]] >> >> _______________________________________________ Bioconductor mailing >> list Bioconductor@stat.math.ethz.ch >> https://stat.ethz.ch/mailman/listinfo/bioconductor Search the >> archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor > [[alternative HTML version deleted]]
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Use affxparser or affyio to read the CEL files - way better than convert binary CELs to text and then use obsolete parser to read them. Cheers Henrik On Tue, Jun 24, 2008 at 8:54 AM, Matthew McCormack <mccormack at="" molbio.mgh.harvard.edu=""> wrote: > You might want to try *CEL File Conversion* Tool found at > http://www.affymetrix.com/support/developer/tools/devnettools.affx. > This is used to . .. "convert all Affymetrix CEL files in a specified > directory from version 3 format (ASCII - MAS 5 compatible format) _to > and from_ version 4 format (binary - GCOS compatible format). You files > may be version 4. If you convert them to version 3, you may have what > you want. It is also possible to convert back to version 4 after some > processing. > > Matthew McCormack > > > James W. MacDonald wrote: >> Hi AA, >> >> A A wrote: >>> Hello Everyone, >>> >>> I am very new to using R and Bioconductor, so please bare with me. I >>> have Affymetrix files that are in CEL format and i am trying to >>> convert them into text files so that I can work with them. I have >>> been trying to use the command justSNPRMA, but I am not sure what to >>> do after I run the command. I tried searching on the net to see if >>> there were step-by-step instructions for doing this, but I have not >>> been successful. Would someone direct me to such a site or explain >>> this process to me? >> >> You might want to look at the posting guide, as this message fails to >> meet even the minimum requirements. Some questions for you: >> >> 1.) What kind of Affymetrix files? >> >> 2.) What does 'work with them' mean? >> >> 3.) What (exact) code have you tried? >> >> 4.) What is your output from sessionInfo()? >> >> 5.) Who are you (anonymous questions are less likely to be answered)? >> >> Best, >> >> Jim >> >> >>> >>> Thanks, >>> >>> AA >>> >>> _________________________________________________________________ >>> [[elided Hotmail spam]] >>> >>> ntrosrchcashback [[alternative HTML version deleted]] >>> >>> _______________________________________________ Bioconductor mailing >>> list Bioconductor at stat.math.ethz.ch >>> https://stat.ethz.ch/mailman/listinfo/bioconductor Search the >>> archives: >>> http://news.gmane.org/gmane.science.biology.informatics.conductor >> > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor >
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cstrato ★ 3.9k
@cstrato-908
Last seen 6.2 years ago
Austria
Affymetrix has a "CEL File Conversion Tool" which converts binary CEL-files to text CEL-files, see: http://www.affymetrix.com/support/developer/tools/devnettools.affx Is this what you are looking for? Christian _._._._._._._._._._._._._._._._ C.h.i.s.t.i.a.n S.t.r.a.t.o.w.a V.i.e.n.n.a A.u.s.t.r.i.a e.m.a.i.l: cstrato at aon.at _._._._._._._._._._._._._._._._ A A wrote: > Hello Everyone, > > I am very new to using R and Bioconductor, so please bare with me. I have Affymetrix files that are in CEL format and i am trying to convert them into text files so that I can work with them. I have been trying to use the command justSNPRMA, but I am not sure what to do after I run the command. I tried searching on the net to see if there were step-by-step instructions for doing this, but I have not been successful. Would someone direct me to such a site or explain this process to me? > > Thanks, > > AA > > _________________________________________________________________ > [[elided Hotmail spam]] > > ntrosrchcashback > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > > >
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Mark Cowley ▴ 400
@mark-cowley-2858
Last seen 9.3 years ago
Australia
More information needed AA, since i've interpreted your question differently to all of the other replies. Do you want to export the RMA processed data, or the raw CEL data in a human readable format? mark ----------------------------------------------------- Mark Cowley, BSc (Bioinformatics)(Hons) Peter Wills Bioinformatics Centre Garvan Institute of Medical Research ----------------------------------------------------- On 24/06/2008, at 9:46 PM, A A wrote: > Hello Everyone, > > I am very new to using R and Bioconductor, so please bare with me. > I have Affymetrix files that are in CEL format and i am trying to > convert them into text files so that I can work with them. I have > been trying to use the command justSNPRMA, but I am not sure what to > do after I run the command. I tried searching on the net to see if > there were step-by-step instructions for doing this, but I have not > been successful. Would someone direct me to such a site or explain > this process to me? > > Thanks, > > AA > > _________________________________________________________________ > [[elided Hotmail spam]] > > ntrosrchcashback > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
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