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Brian Herb
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@brian-herb-3002
Last seen 10.2 years ago
I'm running into a small problem creating the PD package for the bee.
It is
looking for geometry info, but I can't seem to add it.
The package is created with no error, and the XYS files seem to load,
but
returns: Error in geometry(get(pkgname)) :
no slot of name "geometry" for this object of class "platformDesign"
### constructing package
> library(makePlatformDesign)
Loading required package: affyio
Loading required package: oligo
Loading required package: Biobase
Loading required package: tools
Welcome to Bioconductor
Vignettes contain introductory material. To view, type
'openVignette()'. To cite Bioconductor, see
'citation("Biobase")' and for packages 'citation(pkgname)'.
Loading required package: splines
Loading required package: RSQLite
Loading required package: DBI
>
makePDpackage("~/Bee_Project/ApisMel4_JHpkns_AF_meth_HX1.ndf","~/Bee_P
roject/Bee_XYS/247194_635.xys","~/Bee_Project/ApisMel4_JHpkns_AF_meth_
HX1.pos",manufacturer="nimblegen",type="tiling",ncol=14
00,nrow=1050)
Error in
makePDpackage("~/Bee_Project/ApisMel4_JHpkns_AF_meth_HX1.ndf", :
unused argument(s) (ncol = 1400, nrow = 1050)
>
makePDpackage("~/Bee_Project/ApisMel4_JHpkns_AF_meth_HX1.ndf","~/Bee_P
roject/Bee_XYS/247194_635.xys","~/Bee_Project/ApisMel4_JHpkns_AF_meth_
HX1.pos",manufacturer="nimblegen",type="tiling")
nimblegen tiling
The package will be called pd.apismel4.jhpkns.af.meth.hx1
Reading ~/Bee_Project/ApisMel4_JHpkns_AF_meth_HX1.ndf
Assuming a simple design (NOT multiple-wells).
Correcting probe positions... Done.
Creating package in
/home/bst/other/bherb/pd.apismel4.jhpkns.af.meth.hx1
[1] "pd.apismel4.jhpkns.af.meth.hx1"
### Installing package
[bherb@compute-0-43 ~]$ R CMD INSTALL -l ~/bin/R-devel/library
~/pd.apismel4.jhpkns.af.meth.hx1
* Installing *source* package 'pd.apismel4.jhpkns.af.meth.hx1' ...
** R
** data
** help
chmod: cannot access
`/home/bst/other/bherb/bin/R-devel/library/pd.apismel4.jhpkns.af.meth.
hx1/man/pd.apismel4.jhpkns.af.meth.hx1.Rd.gz':
No such file or directory
>>> Building/Updating help pages for package
'pd.apismel4.jhpkns.af.meth.hx1'
Formats: text html latex example
** building package indices ...
Warning in max.default(numeric(0), na.rm = FALSE) :
no non-missing arguments to max; returning -Inf
* DONE (pd.apismel4.jhpkns.af.meth.hx1)
### reading XYS files - based on Preprocessing script - uses
read.xysfiles
command
> source("~/Bee_Project/PreprocessingBee.R")
Loading required package: lattice
Loading required package: Biobase
Loading required package: tools
Welcome to Bioconductor
Vignettes contain introductory material. To view, type
'openVignette()'. To cite Bioconductor, see
'citation("Biobase")' and for packages 'citation(pkgname)'.
Loading required package: affy
Loading required package: affyio
Loading required package: preprocessCore
Loading required package: limma
Loading required package: survival
Loading required package: splines
Loading required package: annotate
Loading required package: AnnotationDbi
Loading required package: DBI
Loading required package: RSQLite
Loading required package: xtable
KernSmooth 2.22 installed
Copyright M. P. Wand 1997
Loading required package: affxparser
Loading required package: oligoClasses
oligo Package - Series 1.5.x
Attaching package: 'oligo'
The following object(s) are masked from package:affy :
list.celfiles,
MAplot,
mm,
mm<-,
mmindex,
pm,
pm<-,
pmindex,
probeNames,
rma
Loading required package: pd.apismel4.jhpkns.af.meth.hx1
Platform design info loaded.
. Done.
. Done.
. Done.
. Done.
. Done.
. Done.
. Done.
. Done.
. Done.
. Done.
. Done.
. Done.
Error in geometry(get(pkgname)) :
no slot of name "geometry" for this object of class "platformDesign"
> traceback()
6: geometry(get(pkgname))
5: geometry(get(pkgname))
4: read.xysfiles(file.path(datapath, pd$Filename), verbose = TRUE,
phenoData = new("AnnotatedDataFrame", data = pd))
3: eval.with.vis(expr, envir, enclos)
2: eval.with.vis(ei, envir)
1: source("~/Bee_Project/PreprocessingBee.R")
> sessionInfo()
R version 2.8.0 Under development (unstable) (2008-03-30 r44996)
x86_64-unknown-linux-gnu
locale:
LC_CTYPE=en_US.iso885915;LC_NUMERIC=C;LC_TIME=en_US.iso885915;LC_COLLA
TE=en_US.iso885915;LC_MONETARY=C;LC_MESSAGES=en_US.iso885915;LC_PAPER=
en_US.iso885915;LC_NAME=C;LC_ADDRESS=C;LC_TELEPHONE=C;LC_MEASUREMENT=e
n_US.iso885915;LC_IDENTIFICATION=C
attached base packages:
[1] splines tools stats graphics grDevices utils
datasets
[8] methods base
other attached packages:
[1] pd.apismel4.jhpkns.af.meth.hx1_1.5.0 oligo_1.5.6
[3] oligoClasses_1.3.4 affxparser_1.13.3
[5] geneplotter_1.19.4 annotate_1.19.2
[7] xtable_1.5-2 AnnotationDbi_1.3.8
[9] RSQLite_0.6-9 DBI_0.2-4
[11] genefilter_1.21.3 survival_2.34-1
[13] vsn_3.7.6 limma_2.15.9
[15] affy_1.19.4 preprocessCore_1.3.2
[17] affyio_1.9.0 Biobase_2.1.7
[19] lattice_0.17-10
loaded via a namespace (and not attached):
[1] grid_2.8.0 KernSmooth_2.22-22 RColorBrewer_1.0-2
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