Hi, I am working on a time course series looking at expression differences between males and females. We expect some genes to be sex- differentially expressed at only some time points, and to show no expression at other time points (i.e have missing values). When I use contrasts.fit to do an ANOVA-like analysis to find genes with sex-differential expression, any gene like this will not be analyzed. However, using the aov function in R, these genes are analyzed. Is there any way to include these genes so they are analyzed when using contrasts.fit? Thanks, -- Matthew Lebo Molecular and Computational Biology University of Southern California [[alternative HTML version deleted]]