Hi, I would like to know if someone has experience with the Illumina Detection Pvalues. Illlumina does not take into account the GC content but just the distribution of a mixture of negative controls. For 50-mers is it not important to account for the GC-content? Is there a threshold for detection that has been verified? Lana Schaffer Biostatistics/Informatics The Scripps Research Institute DNA Array Core Facility La Jolla, CA 92037 (858) 784-2263 (858) 784-2994 schaffer at scripps.edu