Dhiraj, Oligo, oligoClasses and pdInfoBuilder are undergoing some major upgrades for analysis of Nimblegen data (expression and tiling), they likely will not be available with this October release of Bioconductor but will be a part of the development version. In the meantime you can contact me directly and I can provide you the functions you need to get going, such as to read pair files. Matt Settles Bioinformatics Core, Washington State University > Hi, > I am postdoc working with genome wide gene expression using Poplar > oligonucleotide Nimblgen array. I am beginner in R and bioconductor. I have > Nimblgen raw data in .pair file format. I dont know how to use that using > Oligo vignette which is available in bioconductor. How to have input file in > R format from .pair files? > Thanks > Dhriaj > -- > Dhiraj Naik > Postdoctoral Research Associate