Hi to all, I was playing with samr, and I found out that when I try to apply it to some data with no variance, it always return the error message: *"invalid number of intervals"* example of the one dataset: gene_name gene_index 1 1 1 1 -0.719512126 -0.719512126 2 2 -1.2910361 -1.2910361 3 3 -2.503576664 -2.503576664 4 4 -2.595720177 -2.595720177 5 5 0.337146509 0.337146509 6 6 -0.336816551 -0.336816551 7 7 0.463494332 0.463494332 8 8 -0.143871617 -0.143871617 9 9 -0.75348224 -0.75348224 10 10 -0.418839064 -0.418839064 R version 2.7.0 (2008-04-22) i386-pc-mingw32 locale: LC_COLLATE=English_United States.1252;LC_CTYPE=English_United States.1252;LC_MONETARY=English_United States.1252;LC_NUMERIC=C;LC_TIME=English_United States.1252 attached base packages: [1] tools stats graphics grDevices utils datasets methods base other attached packages: [1] samr_1.25 impute_1.12.0 biomarkerDiscovery_0.0.18 annotate_1.18.0 xtable_1.5-2 [6] topGO_1.8.1 SparseM_0.77 GO.db_2.2.0 AnnotationDbi_1.2.0 RSQLite_0.6-8 [11] DBI_0.2-4 graph_1.18.1 limma_2.14.1 affy_1.18.0 preprocessCore_1.2.0 [16] affyio_1.8.0 Biobase_2.0.1 loaded via a namespace (and not attached): [1] cluster_1.11.10 Does any one know if the origin of the problem is the absence of variance? Thanks in advance Regards, Henrique Proen?a