Error in getCdfInfo(object) when using QCReport
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Xingmei Wang ▴ 40
@xingmei-wang-3107
Last seen 11.1 years ago
My code is as following: > setwd("G:/XWang/microarray_phototherapy/CEL file") > library(affyQCReport) Loading required package: Biobase Loading required package: tools Welcome to Bioconductor Vignettes contain introductory material. To view, type 'openVignette()'. To cite Bioconductor, see 'citation("Biobase")' and for packages 'citation(pkgname)'. Loading required package: affy Loading required package: simpleaffy Loading required package: genefilter Loading required package: survival Loading required package: splines Loading required package: gcrma Loading required package: matchprobes Loading required package: xtable Loading required package: affyPLM Loading required package: preprocessCore Loading required package: RColorBrewer Loading required package: geneplotter Loading required package: lattice Loading required package: annotate Loading required package: AnnotationDbi KernSmooth 2.22 installed Copyright M. P. Wand 1997 > mydata<-ReadAffy() > QCReport(mydata, file="GelfandQC.pdf") Error in getCdfInfo(object) : Could not obtain CDF environment, problems encountered: Specified environment does not contain HuGene-1_0-st-v1 Library - package hugene10stv1cdf not installed Bioconductor - hugene10stv1cdf not available Any suggestion would be highly appreciated! Xingmei
cdf cdf • 1.7k views
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@james-w-macdonald-5106
Last seen 10 hours ago
United States
Hi Xingmei, Xingmei Wang wrote: > My code is as following: >> setwd("G:/XWang/microarray_phototherapy/CEL file") >> library(affyQCReport) > Loading required package: Biobase > Loading required package: tools > Welcome to Bioconductor > Vignettes contain introductory material. To view, type > 'openVignette()'. To cite Bioconductor, see > 'citation("Biobase")' and for packages 'citation(pkgname)'. > Loading required package: affy > Loading required package: simpleaffy > Loading required package: genefilter > Loading required package: survival > Loading required package: splines > Loading required package: gcrma > Loading required package: matchprobes > Loading required package: xtable > Loading required package: affyPLM > Loading required package: preprocessCore > Loading required package: RColorBrewer > Loading required package: geneplotter > Loading required package: lattice > Loading required package: annotate > Loading required package: AnnotationDbi > KernSmooth 2.22 installed > Copyright M. P. Wand 1997 >> mydata<-ReadAffy() >> QCReport(mydata, file="GelfandQC.pdf") > Error in getCdfInfo(object) : > Could not obtain CDF environment, problems encountered: > Specified environment does not contain HuGene-1_0-st-v1 > Library - package hugene10stv1cdf not installed > Bioconductor - hugene10stv1cdf not available > Any suggestion would be highly appreciated! The affyQCReport uses the affy package for doing the analysis. Unfortunately, the affy package doesn't support the HuGene chips. To analyze these chips you need to use either oligo or xps. Best, Jim > Xingmei > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician Hildebrandt Lab 8220D MSRB III 1150 W. Medical Center Drive Ann Arbor MI 48109-0646 734-936-8662
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Thanks a lot Jim for your quick response! James W. MacDonald wrote: > Hi Xingmei, > > Xingmei Wang wrote: >> My code is as following: >>> setwd("G:/XWang/microarray_phototherapy/CEL file") >>> library(affyQCReport) >> Loading required package: Biobase >> Loading required package: tools >> Welcome to Bioconductor >> Vignettes contain introductory material. To view, type >> 'openVignette()'. To cite Bioconductor, see >> 'citation("Biobase")' and for packages 'citation(pkgname)'. >> Loading required package: affy >> Loading required package: simpleaffy >> Loading required package: genefilter >> Loading required package: survival >> Loading required package: splines >> Loading required package: gcrma >> Loading required package: matchprobes >> Loading required package: xtable >> Loading required package: affyPLM >> Loading required package: preprocessCore >> Loading required package: RColorBrewer >> Loading required package: geneplotter >> Loading required package: lattice >> Loading required package: annotate >> Loading required package: AnnotationDbi >> KernSmooth 2.22 installed >> Copyright M. P. Wand 1997 >>> mydata<-ReadAffy() >>> QCReport(mydata, file="GelfandQC.pdf") >> Error in getCdfInfo(object) : Could not obtain CDF environment, >> problems encountered: >> Specified environment does not contain HuGene-1_0-st-v1 >> Library - package hugene10stv1cdf not installed >> Bioconductor - hugene10stv1cdf not available >> Any suggestion would be highly appreciated! > > The affyQCReport uses the affy package for doing the analysis. > Unfortunately, the affy package doesn't support the HuGene chips. To > analyze these chips you need to use either oligo or xps. I'm still new in this field, so will need to do some reading on what you suggested. Thanks again! > > Best, > > Jim > > >> Xingmei >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at stat.math.ethz.ch >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor > -- Xingmei wang , MS Biostatistician Biostatistics Analysis Center (BAC) University of Pennsylvania School of Medicine Center for Clinical Epidemiology and Biostatistics 519 Blockley Hall 423 Guardian Drive Philadelphia, PA 19104 Phone: 215-898-1560 Fax: 215-573-1203 Email: xwang5 at mail.med.upenn.edu Website: http://www.cceb.med.upenn.edu/services/bac/
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