I'm trying to plot background intensity after clean empty values. When I check the information through layout, I got ngrid.r=12, ngrid.c=4, nspot.r=25, nspot.c=24 (28800 spots) Cleaning empty data I have 27003 spots., searching in forum I found this suggestion: Dear Vanessa, > > limma always assumes complete print-tip-groups, so the only way to > use imageplot() correctly is to complete your arrays by putting back > > to last 4 spots of each print-tip-group as NA. You can do this by: > > narrays <- ncol(RG_e1) > Y <- matrix(NA,21632,narrays) > RG <- new("RGList",list(R=Y,G=Y,Rb=Y,Gb=Y) > RG$printer <- RG_e1$printer > > missingspots <- spotr(RG$printer)==26 & spotc(RG$printer)>=23 > RG$R[!i,] <- RG_e1$R > RG$Rb[!i,] <- RG_e1$Rb > RG$G[!i,] <- RG_e1$G > RG$Gb[!i,] <- RG_e1$Gb > > Best wishes > > Gordon > > but If i try to follow the commands I have this error message: missingspots <- spotr(RG1$printer)==26 & spotc(RG1$printer)>=23 Error en 1:layout$nspot.r : Argumento NA/NaN Can anyone suggest me something? Thanks -- José Antonio Coria Fernández Universidad Nacional Autonóma de México Facultad de Estudios Superiores Acatlán Centro de Desarrollo Tecnológico (Departamento Servicios de Cómputo) Av. Alcanfores y San Juan Totoltepec S/N C.P. 53150 Tel: 56 23 17 62, 56 23 15 07 Cubiculo 114 Planta Alta email: com90185@gmail.com, jcoriafdz@hotmail.com (_)(_) (,,) =()= ((__)\ _|L\_______/ The Lab Rat [[alternative HTML version deleted]]