CellHTS Replicates Missing
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@becky-saunders-2923
Last seen 10.3 years ago
Hello there, Sorry if this question has already been answered. I have been using CellHTS2 for a while now but have recently upgraded to R version 2.9.1 and CellHTS2 version 2.8.1. Using my previous version I could miss replicates from the plate list file without generating an error - for example : Filename Plate Replicate plate_1_1.txt 1 1 plate_1_2.txt 1 2 plate_1_3.txt 1 3 plate_2_1.txt 2 1 plate_2_2.txt 2 2 plate_2_3.txt 2 3 plate_3_2.txt 3 2 plate_3_3.txt 3 3 Where plate 3, replicate 1 is missing. However, with my current version this generates the following error: Error in data.frame(title = title, shortTitle = title, thumbnail = img, : arguments imply differing number of rows: 3, 2 . I have tried it also when missing replicate 2 and replicate 3 and I still get the error. Can I still analyse plates with a different number of replicates? All the plate data seems to be fine and when I include all 3 replicates I do not get any errors. Many Thanks, Becky Saunders -- Dr Rebecca Saunders High Throughput Screening Laboratory - Cancer Research UK Tel No. +44 (0)207 269 3159 Fax No. +44 (0)207 269 3581 This communication is from Cancer Research UK. Our website is at www.cancerresearchuk.org. We are a charity registered under number 1089464 and a company limited by guarantee registered in England & Wales under number 4325234. Our registered address is 61 Lincoln's Inn Fields, London WC2A 3PX. Our central telephone number is 020 7242 0200. This communication and any attachments contain information which is confidential and may also be privileged. It is for the exclusive use of the intended recipient(s). If you are not the intended recipient(s) please note that any form of disclosure, distribution, copying or use of this communication or the information in it or in any attachments is strictly prohibited and may be unlawful. If you have received this communication in error, please notify the sender and delete the email and destroy any copies of it. E-mail communications cannot be guaranteed to be secure or error free, as information could be intercepted, corrupted, amended, lost, destroyed, arrive late or incomplete, or contain viruses. We do not accept liability for any such matters or their consequences. Anyone who communicates with us by e-mail is taken to accept the risks in doing so. [[alternative HTML version deleted]]
Cancer cellHTS2 Cancer cellHTS2 • 1.4k views
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Florian Hahne ▴ 540
@florian-hahne-2471
Last seen 10.3 years ago
Hi Becky, missing replicates should still work, so this is definitely a bug. Could you send me all the files needed to reproduce the problem, and also all the code. I will fix this asap and will let you know once the new version is available. Florian Becky Saunders wrote: > Hello there, > > > > Sorry if this question has already been answered. > > > > I have been using CellHTS2 for a while now but have recently upgraded to > R version 2.9.1 and CellHTS2 version 2.8.1. > > > > Using my previous version I could miss replicates from the plate list > file without generating an error - for example : > > > > Filename Plate Replicate > > plate_1_1.txt 1 1 > > plate_1_2.txt 1 2 > > plate_1_3.txt 1 3 > > plate_2_1.txt 2 1 > > plate_2_2.txt 2 2 > > plate_2_3.txt 2 3 > > plate_3_2.txt 3 2 > > plate_3_3.txt 3 3 > > > > Where plate 3, replicate 1 is missing. However, with my current version > this generates the following error: > > > > Error in data.frame(title = title, shortTitle = title, thumbnail = img, > : arguments imply differing number of rows: 3, 2 . > > > > I have tried it also when missing replicate 2 and replicate 3 and I > still get the error. > > > > Can I still analyse plates with a different number of replicates? > > > > All the plate data seems to be fine and when I include all 3 replicates > I do not get any errors. > > > > Many Thanks, > > > > Becky Saunders > > > > > > -- > > Dr Rebecca Saunders > > High Throughput Screening Laboratory - Cancer Research UK > > Tel No. +44 (0)207 269 3159 > > Fax No. +44 (0)207 269 3581 > > > > > This communication is from Cancer Research UK. Our website is at www.cancerresearchuk.org. We are a charity registered under number 1089464 and a company limited by guarantee registered in England & Wales under number 4325234. Our registered address is 61 Lincoln's Inn Fields, London WC2A 3PX. Our central telephone number is 020 7242 0200. > > This communication and any attachments contain information which is confidential and may also be privileged. It is for the exclusive use of the intended recipient(s). If you are not the intended recipient(s) please note that any form of disclosure, distribution, copying or use of this communication or the information in it or in any attachments is strictly prohibited and may be unlawful. If you have received this communication in error, please notify the sender and delete the email and destroy any copies of it. > > E-mail communications cannot be guaranteed to be secure or error free, as information could be intercepted, corrupted, amended, lost, destroyed, arrive late or incomplete, or contain viruses. We do not accept liability for any such matters or their consequences. Anyone who communicates with us by e-mail is taken to accept the risks in doing so. > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > -- Florian Hahne, PhD Computational Biology Program Division of Public Health Sciences Fred Hutchinson Cancer Research Center 1100 Fairview Ave. N, M1-B514 PO Box 19024 Seattle, Washington 98109-1024 206-667-3148 fhahne at fhcrc.org
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