Array express looking in wrong place for file...
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Paul Geeleher ★ 1.3k
@paul-geeleher-2679
Last seen 10.2 years ago
Hi I'm getting this error when I try to dowload from ArrayExpress: mRnaData <- ArrayExpress("E-UCON-1") ... In getAE(input, path, type = "raw") : http://www.ebi.ac.uk/microarray-as/ae/files/E-UCON-1/E-UCON-1.raw.zip does not exist or is empty. ... The thing is its looking in the wrong place for this file, if I go to the array express website the files does exist but is at: http://www.ebi.ac.uk/microarray- as/ae/files/E-UCON-1/E-UCON-1.raw.1.zip Note the 1 after the word raw... Any way to specify this address manually? Or another solution? I haven't been able to find one. Thanks alot -Paul. -- Paul Geeleher School of Mathematics, Statistics and Applied Mathematics National University of Ireland Galway Ireland [[alternative HTML version deleted]]
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audrey ▴ 280
@audrey-2551
Last seen 10.2 years ago
Hi Paul, I apologize for the delay in replying. The ArrayExpress database has been completely regenerated two weeks ago and some changes (like the naming of the zip files) needed some time to get synchronized with the Bioconductor package (especially because I was away at the same moment). The problem of the file names is solved in ArrayExpress version 1.5.11 and future versions. If you update the package (using the development version or use the new release when it is available), it should work fine. Please note that for this particular dataset, you will need first to download the data using getAE() function and then set the column names in the function magetab2bioc() as the scanner column names are apparently not part of the known quantitation types from ArrayExpress: library("ArrayExpress") EUCON1 = getAE("E-UCON-1") mRnaData = magetab2bioc(EUCON1, rawcol = list(R="Feature Extraction Software:rMeanSignal", G="Feature Extraction Software:gMeanSignal", Rb="Feature Extraction Software:rBGMeanSignal", Gb="Feature Extraction Software:gBGMeanSignal" )) Sorry again for the problem and the delay replying, Audrey Paul Geeleher wrote: > Hi I'm getting this error when I try to dowload from ArrayExpress: > > mRnaData <- ArrayExpress("E-UCON-1") > ... > In getAE(input, path, type = "raw") : > http://www.ebi.ac.uk/microarray- as/ae/files/E-UCON-1/E-UCON-1.raw.zip does > not exist or is empty. > ... > > The thing is its looking in the wrong place for this file, if I go to the > array express website the files does exist but is at: > > http://www.ebi.ac.uk/microarray- as/ae/files/E-UCON-1/E-UCON-1.raw.1.zip > > Note the 1 after the word raw... > > Any way to specify this address manually? Or another solution? I haven't > been able to find one. > > Thanks alot > > -Paul. > > > -- Audrey Kauffmann EMBL - EBI Cambridge UK +44 (0) 1223 492 631 http://www.ebi.ac.uk/~audrey
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