Dear List,
I'm attempting my first microarray analysis on a methylation dataset
(Illumina Infinium) comprising identical twins (24 -> 12 per array). I
thought I'd try the methylumi package - I can import the data, but
when
I try to Normalise between the two arrays I get an error:
> normalizeMethyLumiSet(meth)
Error in function (classes, fdef, mtable) :
unable to find an inherited method for function "QCdata<-", for
signature "MethyLumiSet", "NULL"
According to the vignette, QCdata is not required.
I know some basics but am not an expert in R.
Any help would be appreciated.
Thank you.
Paul Sanfilippo
On Thu, Jan 14, 2010 at 10:45 PM, Paul Sanfilippo <prseye at="" gmail.com=""> wrote:
> Dear List,
>
> I'm attempting my first microarray analysis on a methylation dataset
> (Illumina Infinium) comprising identical twins (24 -> 12 per array).
I
> thought I'd try the methylumi package - I can import the data, but
when I
> try to Normalise between the two arrays I get an error:
>
>> normalizeMethyLumiSet(meth)
> Error in function (classes, fdef, mtable) ?:
> ?unable to find an inherited method for function "QCdata<-", for
signature
> "MethyLumiSet", "NULL"
>
> According to the vignette, QCdata is not required.
>
> I know some basics but am not an expert in R.
>
> Any help would be appreciated.
Paul, could you do me a quick favor and send along the output of
sessionInfo()?
Thanks,
Sean
This is the output:
> sessionInfo()
R version 2.10.1 Patched (2009-12-29 r50852)
x86_64-apple-darwin9.8.0
locale:
[1] C/C/en_US/C/C/C
attached base packages:
[1] grid stats graphics grDevices utils datasets
methods
[8] base
other attached packages:
[1] methylumi_1.0.0 Biobase_2.6.1 ggplot2_0.8.5 digest_0.4.2
[5] reshape_0.8.3 plyr_0.1.9 proto_0.3-8 JGR_1.7-0
[9] iplots_1.1-3 JavaGD_0.5-2 rJava_0.8-1
loaded via a namespace (and not attached):
[1] lattice_0.17-26 tools_2.10.1
Thanks Sean.
Paul
Sean Davis wrote:
> On Thu, Jan 14, 2010 at 10:45 PM, Paul Sanfilippo<prseye@gmail.com>
wrote:
>
>> Dear List,
>>
>> I'm attempting my first microarray analysis on a methylation
dataset
>> (Illumina Infinium) comprising identical twins (24 -> 12 per
array). I
>> thought I'd try the methylumi package - I can import the data, but
when I
>> try to Normalise between the two arrays I get an error:
>>
>>
>>> normalizeMethyLumiSet(meth)
>>>
>> Error in function (classes, fdef, mtable) :
>> unable to find an inherited method for function "QCdata<-", for
signature
>> "MethyLumiSet", "NULL"
>>
>> According to the vignette, QCdata is not required.
>>
>> I know some basics but am not an expert in R.
>>
>> Any help would be appreciated.
>>
>
> Paul, could you do me a quick favor and send along the output of
sessionInfo()?
>
> Thanks,
> Sean
>
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