Entering edit mode
Frederico Arnoldi
▴
40
@frederico-arnoldi-4001
Last seen 10.2 years ago
Dear list,
I am having problems with the "spillover" method of the flowCore
package. I am trying to compensate a flowset but everytime I tried to
get the spillover matrix from my control data, I received the
following error message:
"Error in .local(x, ...) : subscript out of bounds"
In my flowset I have 6 flowframes (in the following order: unstained
cells, APC, PercPCy5.5, FITC, PeCy7, PE) that I have loaded as
following:
>library("flowCore")
>library("flowViz")
>fs = read.flowSet(path = "path")
>fs
A flowSet with 6 experiments.
column names:
FSC-H SSC-A FITC-A PE-A PerCP-A PE Cy7-A APC-A Time
I tried to get the spillover matrix with
> comp.mat <- spillover(fs, unstained=1, fsc = "FSC-H", ssc= "SSC-A")
Error in .local(x, ...) : subscript out of bounds
I can not identify where is my mistake. I tried a fast look in the
source code of "spillover method" (starting at line 525 of the
flowSet-accessors.R in the flowCore_1.8.3.tar.gz), but I also not able
to identify the source of my error or a possible bug.
Please, could anyone help me with it?
Thanks in advance,
Fred
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