Dear Varpal, You can apply the fold change cutoff by ranking the various contrasts by M in the AffylmGUI top table menu and implementing the cutoff in excel. I hope this help, Rich ------------------------------------------------------------ Richard A. Friedman, PhD Associate Research Scientist, Biomedical Informatics Shared Resource Herbert Irving Comprehensive Cancer Center (HICCC) Lecturer, Department of Biomedical Informatics (DBMI) Educational Coordinator, Center for Computational Biology and Bioinformatics (C2B2)/ National Center for Multiscale Analysis of Genomic Networks (MAGNet) Room 824 Irving Cancer Research Center Columbia University 1130 St. Nicholas Ave New York, NY 10032 (212)851-4765 (voice) friedman at cancercenter.columbia.edu http://cancercenter.columbia.edu/~friedman/ In Memoriam, Philip Klass On Apr 9, 2010, at 6:02 AM, varpal singh wrote: > Dear Madam/Sir, > > I have used affylmGUI for time series experiment, I want to apply fold > change cut-off.. > How can I do thiss on affyl;mGUI ?????? > > Thanking you > Varpal > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor