I apologize for asking again the same question I asked some months ago. The reason is that a question has been raised recently by people I work with. The question stemmed from the double meaning of the word "validated" with reference to miRNAs. Some time ago I downloaded the Fasta files "mature.fa" and "matureStart.fa" (the latter cannot be found any more). I meant to get miRNAs that have been experimentally validated against (binding to) some gene targets. I used the dat set "hsTargets" avaiilable with Bioconductor to find the matching miRNA-terget pairs. The question arisen is: "are the nmiRNAs I got just predicted by some code (miRanda or the like) or have they been experimentally validated" ? I would appreciate your comments. Thank you in advance Maura tutti i telefonini TIM! [[alternative HTML version deleted]]

Tarbase and miRecords are the only databases of validated targets I know of. MirWalk has a validated section but I think it comes from text mining. ________________________________________ From: bioconductor-bounces@stat.math.ethz.ch [bioconductor- bounces@stat.math.ethz.ch] On Behalf Of mauede@alice.it [mauede@alice.it] Sent: 01 May 2010 06:11 To: Bioconductor List Subject: [BioC] Validated miRNAs revisited I apologize for asking again the same question I asked some months ago. The reason is that a question has been raised recently by people I work with. The question stemmed from the double meaning of the word "validated" with reference to miRNAs. Some time ago I downloaded the Fasta files "mature.fa" and "matureStart.fa" (the latter cannot be found any more). I meant to get miRNAs that have been experimentally validated against (binding to) some gene targets. I used the dat set "hsTargets" avaiilable with Bioconductor to find the matching miRNA-terget pairs. The question arisen is: "are the nmiRNAs I got just predicted by some code (miRanda or the like) or have they been experimentally validated" ? I would appreciate your comments. Thank you in advance Maura tutti i telefonini TIM! [[alternative HTML version deleted]] _______________________________________________ Bioconductor mailing list Bioconductor at stat.math.ethz.ch https://stat.ethz.ch/mailman/listinfo/bioconductor Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor