Dear Madam/Sir,, I have just two CEL files one from wild and other from treated. I want to ask I have to need for considering design and contrast matrix in bioconductor for identifing differential gene expression data ? Thanking you varpall [[alternative HTML version deleted]]

On Tue, May 4, 2010 at 10:16 AM, varpal singh <gilvarpal at="" gmail.com=""> wrote: > Dear Madam/Sir,, > > ?I have just two CEL files one from wild and other from treated. I want to > ask I have to need for considering design and contrast matrix in > bioconductor for identifing differential gene expression data ? Hi, Varpall. Unfortunately, you cannot perform a statistical test between two arrays. You could make a ratio between the two and rank genes by that, but there is no rational cutoff or estimate of statistical significance that can be gleaned from this process. If you or your collaborator wants to know an answer, the best bet is to perform some biological replicates. Sean