DNA-Methylation, CopyNumber Results
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Noemi Andor ▴ 100
@noemi-andor-4128
Last seen 10.2 years ago
Hi, I need to parse some raw Methylation data from Illumina (Infinium) and some copy number results (CGH, high amount of data). I would be grateful for any useful information like which bioconducter packages to use, alternative tools, normalization. thank's in advance, Noemi
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Chao-Jen Wong ▴ 580
@chao-jen-wong-3603
Last seen 9.9 years ago
USA/Seattle/Fred Hutchinson Cancer Reseā€¦
As for Illumina Infinium methylation data, you can use the methylumi package to manipulate the data and perform quality control and normalization. Subsequently use limma to do differential methylation analysis. On 06/16/10 19:22, Noemi Andor wrote: > Hi, > > I need to parse some raw Methylation data from Illumina (Infinium) and some copy number results (CGH, high amount of data). I would be grateful for any useful information like which bioconducter packages to use, alternative tools, normalization. > > thank's in advance, > > Noemi > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > -- Chao-Jen Wong Program in Computational Biology Division of Public Health Sciences Fred Hutchinson Cancer Research Center 1100 Fairview Avenue N., M1-B514 PO Box 19024 Seattle, WA 98109 206.667.4485 cwon2 at fhcrc.org
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On Thu, Jun 17, 2010 at 4:43 PM, Chao-Jen Wong <cwon2@fhcrc.org> wrote: > As for Illumina Infinium methylation data, you can use the methylumi > package to manipulate the data and perform quality control and > normalization. Subsequently use limma to do differential methylation > analysis. > > Just keep in mind that the data from the Illumina arrays are pretty non-normal, so some assumptions that come into play when using a parametric testing method may be in question. Sean > On 06/16/10 19:22, Noemi Andor wrote: > > Hi, > > > > I need to parse some raw Methylation data from Illumina (Infinium) and > some copy number results (CGH, high amount of data). I would be grateful for > any useful information like which bioconducter packages to use, alternative > tools, normalization. > > > > thank's in advance, > > > > Noemi > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor@stat.math.ethz.ch > > https://stat.ethz.ch/mailman/listinfo/bioconductor > > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > > > > > -- > Chao-Jen Wong > Program in Computational Biology > Division of Public Health Sciences > Fred Hutchinson Cancer Research Center > 1100 Fairview Avenue N., M1-B514 > PO Box 19024 > Seattle, WA 98109 > 206.667.4485 > cwon2@fhcrc.org > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > [[alternative HTML version deleted]]
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