Hello everyone ... i am trying to make an object from an SMD data downloaded from ArrayExpress ... using the limma package and used the following command .... but getting this error and the object is not being created .. data <- read.maimages(files=filelist, source="smd", columns=list(R="ScanAlyze:RESULT.CH1I_MEAN",G="ScanAlyze:RESULT.CH2I_M EAN",Rb="ScanAlyze:RESULT.CH1B_MEDIAN",Gb="ScanAlyze:RESULT.CH2B_MEDIA N")) Read E-SMDB-1428-raw-data-516957410.txt Read E-SMDB-1428-raw-data-516957425.txt Read E-SMDB-1428-raw-data-516957442.txt Read E-SMDB-1428-raw-data-516957457.txt Read E-SMDB-1428-raw-data-516957472.txt Read E-SMDB-1428-raw-data-516957490.txt Read E-SMDB-1428-raw-data-516957507.txt Error in RG[[a]][, i] <- obj[, columns[[a]]] : number of items to replace is not a multiple of replacement length Please help to sort this problem Regard rohit -- Rohit Farmer M.Tech Bioinformatics Department of Computational Biology and Bioinformatics Jacob School of Biengineering and Biotechnology Sam Higginbottom Institute of Agriculture, Technology and Sciences (Formerly known as Allahabad Agricultural Institute - Deemed University) Allahabad, UP, INDIA - 211 007 Ph. No. 9839845093, 9415261403 e-Mail rohit.farmer@gmail.com Blog http://rohitsspace.blogspot.com [[alternative HTML version deleted]]

Hello everyone ... i am trying to make an object from an SMD data downloaded from ArrayExpress ... using the limma package .. but getting this error and the object is not being created ...... number of items to replace is not a multiple of replacement length. The problem appears to be that data files (total 16 files) have different numbers of rows. All the files have the same number of rows (5337 rows), but only the 8th file is different (4993 rows). how these rows can be made similar in number so that all files can be changed to a single object. how can i deal with this situation. if we read files (1-7 & 9-16) and 8 separately then two objects will be created then how can these two objects be merged for further analysis. Please help to sort this problem Regard -- BUDHAYASH