Hi David, IMHO, changing the meta data in the FC file is outside the intended functionality of flowcore. I would even argue that it is a bad idea. On the other hand, modifying the annotated dataframe associated with a flowSet would be the intended use of flowCore. Here is some sample code for that purpose: adf = pData(aFlowSet) adf$patient_id <- 100 pData(aFlowSet) <- adf or pData(aFlowSet)$patient_id <- 100 ## from the manual pData, pData<- Extract or replace the data frame (or columns thereof) containing actual pheno- typic information from the phenoData slot. Usage: pData(flowSet) pData(flowSet)$someColumn <- value ## Hope that helps, Perry >Message: 10 >Date: Thu, 24 Jun 2010 14:25:38 -0700 (PDT) >From: David Lyon <david_lyon3 at="" yahoo.com=""> >To: bioconductor at stat.math.ethz.ch >Subject: [BioC] flowcore >Message-ID: <224578.300.qm at web113519.mail.gq1.yahoo.com> >Content-Type: text/plain; charset=us-ascii >Hello >can someone show me how to add metadata to a FC file eg: >If I wanted to add $patient_id = 100 to an existing FC file whats the exact code to do this? >also if I wanted to modify an existing value whats the exact code ? >Thanks alot Perry Haaland, Ph.D. BD Fellow, Bioinformatics BD Technologies (919)-349-2172 perry_haaland at bd.com ----------------------------------------- ******************************************************************* IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.: This message may constitute an advertisement of a BD group's products or services or a solicitation of interest in them. If this is such a message and you would like to opt out of receiving future advertisements or solicitations from this BD group, please forward this e-mail to optoutbygroup at bd.com. ******************************************************************* This message (which includes any attachments) is intended only for the designated recipient(s). It may contain confidential or proprietary information and may be subject to the attorney-client privilege or other confidentiality protections. If you are not a designated recipient, you may not review, use, copy or distribute this message. If you received this in error, please notify the sender by reply e-mail and delete this message. Thank you. ******************************************************************* Corporate Headquarters Mailing Address: BD (Becton, Dickinson and Company) 1 Becton Drive Franklin Lakes, NJ 07417 U.S.A.