> > We are looking at some HG-U133A arrays in which the RNA degredation plot > shows a large increase from 5' to 3'. Is it neccessarily bad? Is there a > condition on the slope that defines "bad"? > > Here are the slopes and pvs. > > slope 1.88 1.34 1.86 1.71 > pvalue 1.94e-07 3.74e-05 1.14e-07 6.48e-07 > > Any advice, or pointers to references would be appreciated. >From what I have seen, slopes like this are typical of 133 chips. -Leslie cope