Dear list,
I have a short question: I'm performing heatmaps on lists of genes
selected as being differentially expressed on a set of 4 technical
replicates (from an Agilent gene expression 2 colors experiment).
As technical replicates are behaving very similarly, I am taking the
median values of the replicates log2ratios, and basically trying to
get a heatmaps that would display only one column, being the median
value, and the genes on lines.
I'm using heatmap.2 which doesn't draw heatmaps for data containing
less than 2 columns and 2 rows, so I was wondering if there was a
function which does this?
I know that it doesn't make so much sense, especially not using
biological replicates, but it has a more "visual" goal!
Thank you in advance!
Sarah
You can trick it by replicating the single column/vector to a matrix
of two columns
containing the same values. The result in the heatmap will look like a
single
column.
Thomas
On Wed, Jul 28, 2010 at 11:04:01AM +0200, Sarah Bonnin wrote:
> Dear list,
>
> I have a short question: I'm performing heatmaps on lists of genes
selected as being differentially expressed on a set of 4 technical
replicates (from an Agilent gene expression 2 colors experiment).
>
> As technical replicates are behaving very similarly, I am taking the
median values of the replicates log2ratios, and basically trying to
get a heatmaps that would display only one column, being the median
value, and the genes on lines.
>
> I'm using heatmap.2 which doesn't draw heatmaps for data containing
less than 2 columns and 2 rows, so I was wondering if there was a
function which does this?
>
> I know that it doesn't make so much sense, especially not using
biological replicates, but it has a more "visual" goal!
>
> Thank you in advance!
>
> Sarah
>
>
>
> _______________________________________________
> Bioconductor mailing list
> Bioconductor at stat.math.ethz.ch
> https://stat.ethz.ch/mailman/listinfo/bioconductor
> Search the archives:
http://news.gmane.org/gmane.science.biology.informatics.conductor
>
On Wed, Jul 28, 2010 at 5:04 AM, Sarah Bonnin <sbonnin@crg.es> wrote:
> Dear list,
>
> I have a short question: I'm performing heatmaps on lists of genes
selected
> as being differentially expressed on a set of 4 technical replicates
(from
> an Agilent gene expression 2 colors experiment).
>
> As technical replicates are behaving very similarly, I am taking the
median
> values of the replicates log2ratios, and basically trying to get a
heatmaps
> that would display only one column, being the median value, and the
genes on
> lines.
>
> I'm using heatmap.2 which doesn't draw heatmaps for data containing
less
> than 2 columns and 2 rows, so I was wondering if there was a
function which
> does this?
>
> I know that it doesn't make so much sense, especially not using
biological
> replicates, but it has a more "visual" goal!
>
>
I'd consider using a simple bar chart or a waterfall plot.
Sean
[[alternative HTML version deleted]]
Hi,
how about something like:
> mat = t(matrix(rnorm(100)))
> image(mat)
needs some tuning of colors and axis labels of course. see ?image
Cheers
Michael
Am Jul 28, 2010 um 6:09 PM schrieb Sean Davis:
> On Wed, Jul 28, 2010 at 5:04 AM, Sarah Bonnin <sbonnin at="" crg.es="">
wrote:
>
>> Dear list,
>>
>> I have a short question: I'm performing heatmaps on lists of genes
selected
>> as being differentially expressed on a set of 4 technical
replicates (from
>> an Agilent gene expression 2 colors experiment).
>>
>> As technical replicates are behaving very similarly, I am taking
the median
>> values of the replicates log2ratios, and basically trying to get a
heatmaps
>> that would display only one column, being the median value, and the
genes on
>> lines.
>>
>> I'm using heatmap.2 which doesn't draw heatmaps for data containing
less
>> than 2 columns and 2 rows, so I was wondering if there was a
function which
>> does this?
>>
>> I know that it doesn't make so much sense, especially not using
biological
>> replicates, but it has a more "visual" goal!
>>
>>
> I'd consider using a simple bar chart or a waterfall plot.
>
> Sean
>
> [[alternative HTML version deleted]]
>
> _______________________________________________
> Bioconductor mailing list
> Bioconductor at stat.math.ethz.ch
> https://stat.ethz.ch/mailman/listinfo/bioconductor
> Search the archives:
http://news.gmane.org/gmane.science.biology.informatics.conductor