omitted rows using getBM
1
0
Entering edit mode
@mcmahon-kevin-3198
Last seen 10.2 years ago
Hello everyone, I'm trying to use biomaRt to simply get the Entrez Gene IDs from a list of probe ids from the Affymetrix Mouse 430 2.0 chip. When I use the getBM function, I end up getting a different number of rows in the resulting table than I input. I've read the getBM help and searched this mailing list, but to no avail. My code is below: >mart<-useMart("ensembl", dataset="mmusculus_gene_ensembl") >genetable1239<-getBM(attributes=c("affy_mouse430_2","entrezgene"), values=gds1239[,1], filters="affy_mouse430_2", mart=mart) >dim(gds1239) [1] 45101 2 > dim(genetable1239) [1] 43488 2 > length(unique(gds1239[,1])) [1] 45101 Can anyone help me? Thanks so much, Wyatt > sessionInfo() R version 2.11.1 (2010-05-31) i386-pc-mingw32 locale: [1] LC_COLLATE=English_United States.1252 LC_CTYPE=English_United States.1252 LC_MONETARY=English_United States.1252 LC_NUMERIC=C [5] LC_TIME=English_United States.1252 attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] GEOquery_2.13.5 Biobase_2.8.0 biomaRt_2.4.0 loaded via a namespace (and not attached): [1] RCurl_1.4-2 tools_2.11.1 XML_3.1-0 K. Wyatt McMahon, Ph.D. Postdoctoral Research Associate Department of Internal Medicine 3601 4th St. - Lubbock, TX - 79430 P: 806-743-4072 F: 806-743-3148 [[alternative HTML version deleted]]
probe biomaRt probe biomaRt • 1.2k views
ADD COMMENT
0
Entering edit mode
Steffen ▴ 500
@steffen-2351
Last seen 10.2 years ago
Hi Wyatt, Ensembl does it's own mapping of affy ids to the genome. If they decide there is no good match for an affy probe to the genome then they won't provide any result for that probe, which explains the reduced number of rows. Cheers, Steffen On Wed, Oct 13, 2010 at 2:32 PM, Mcmahon, Kwyatt <kwyatt.mcmahon@ttuhsc.edu>wrote: > Hello everyone, > > I'm trying to use biomaRt to simply get the Entrez Gene IDs from a list of > probe ids from the Affymetrix Mouse 430 2.0 chip. When I use the getBM > function, I end up getting a different number of rows in the resulting table > than I input. I've read the getBM help and searched this mailing list, but > to no avail. My code is below: > > >mart<-useMart("ensembl", dataset="mmusculus_gene_ensembl") > >genetable1239<-getBM(attributes=c("affy_mouse430_2","entrezgene"), > values=gds1239[,1], filters="affy_mouse430_2", mart=mart) > >dim(gds1239) > [1] 45101 2 > > dim(genetable1239) > [1] 43488 2 > > length(unique(gds1239[,1])) > [1] 45101 > > Can anyone help me? > > Thanks so much, > > Wyatt > > > sessionInfo() > R version 2.11.1 (2010-05-31) > i386-pc-mingw32 > > locale: > [1] LC_COLLATE=English_United States.1252 LC_CTYPE=English_United > States.1252 LC_MONETARY=English_United States.1252 LC_NUMERIC=C > [5] LC_TIME=English_United States.1252 > > attached base packages: > [1] stats graphics grDevices utils datasets methods base > > other attached packages: > [1] GEOquery_2.13.5 Biobase_2.8.0 biomaRt_2.4.0 > > loaded via a namespace (and not attached): > [1] RCurl_1.4-2 tools_2.11.1 XML_3.1-0 > > > > K. Wyatt McMahon, Ph.D. > Postdoctoral Research Associate > Department of Internal Medicine > 3601 4th St. - Lubbock, TX - 79430 > P: 806-743-4072 > F: 806-743-3148 > > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > [[alternative HTML version deleted]]
ADD COMMENT
0
Entering edit mode
Thanks so much, Steffen; that answers my question perfectly. Wyatt K. Wyatt McMahon, Ph.D. Postdoctoral Research Associate Department of Internal Medicine 3601 4th St. - Lubbock, TX - 79430 P: 806-743-4072 F: 806-743-3148 From: Steffen Durinck [mailto:sdurinck@lbl.gov] Sent: Wednesday, October 13, 2010 4:40 PM To: Mcmahon, Kwyatt Cc: bioconductor@stat.math.ethz.ch Subject: Re: [BioC] omitted rows using getBM Hi Wyatt, Ensembl does it's own mapping of affy ids to the genome. If they decide there is no good match for an affy probe to the genome then they won't provide any result for that probe, which explains the reduced number of rows. Cheers, Steffen On Wed, Oct 13, 2010 at 2:32 PM, Mcmahon, Kwyatt <kwyatt.mcmahon@ttuhsc.edu<mailto:kwyatt.mcmahon@ttuhsc.edu>> wrote: Hello everyone, I'm trying to use biomaRt to simply get the Entrez Gene IDs from a list of probe ids from the Affymetrix Mouse 430 2.0 chip. When I use the getBM function, I end up getting a different number of rows in the resulting table than I input. I've read the getBM help and searched this mailing list, but to no avail. My code is below: >mart<-useMart("ensembl", dataset="mmusculus_gene_ensembl") >genetable1239<-getBM(attributes=c("affy_mouse430_2","entrezgene"), values=gds1239[,1], filters="affy_mouse430_2", mart=mart) >dim(gds1239) [1] 45101 2 > dim(genetable1239) [1] 43488 2 > length(unique(gds1239[,1])) [1] 45101 Can anyone help me? Thanks so much, Wyatt > sessionInfo() R version 2.11.1 (2010-05-31) i386-pc-mingw32 locale: [1] LC_COLLATE=English_United States.1252 LC_CTYPE=English_United States.1252 LC_MONETARY=English_United States.1252 LC_NUMERIC=C [5] LC_TIME=English_United States.1252 attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] GEOquery_2.13.5 Biobase_2.8.0 biomaRt_2.4.0 loaded via a namespace (and not attached): [1] RCurl_1.4-2 tools_2.11.1 XML_3.1-0 K. Wyatt McMahon, Ph.D. Postdoctoral Research Associate Department of Internal Medicine 3601 4th St. - Lubbock, TX - 79430 P: 806-743-4072 F: 806-743-3148 [[alternative HTML version deleted]] _______________________________________________ Bioconductor mailing list Bioconductor@stat.math.ethz.ch<mailto:bioconductor@stat.math.ethz.ch> https://stat.ethz.ch/mailman/listinfo/bioconductor Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor [[alternative HTML version deleted]]
ADD REPLY

Login before adding your answer.

Traffic: 636 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6