Dear all, I am working with the MEDIPS package and get the following error when calling the function MEDIPS.CpGenrich: > cpgEnrichment_barr<-MEDIPS.CpGenrich(data=barr,extend=fraglen) Preprocessing... Error in .local(x, width, fix, use.names, ...) : unused argument(s) (start = FALSE) Calls: MEDIPS.CpGenrich -> resize -> resize -> .local I checked the resize function which indeed does not require a "start" argument, the function call in MEDIPS.CpGenrich however looks like this: ranges(pos2) <- resize(ranges(pos2), extend + 1, start = FALSE) This sounds like a version problem. This is my sessionInfo: R version 2.11.1 (2010-05-31) x86_64-unknown-linux-gnu locale: [1] LC_CTYPE=en_GB.UTF-8 LC_NUMERIC=C [3] LC_TIME=en_GB.UTF-8 LC_COLLATE=en_GB.UTF-8 [5] LC_MONETARY=C LC_MESSAGES=en_GB.UTF-8 [7] LC_PAPER=en_GB.UTF-8 LC_NAME=C [9] LC_ADDRESS=C LC_TELEPHONE=C [11] LC_MEASUREMENT=en_GB.UTF-8 LC_IDENTIFICATION=C attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] BSgenome.Hsapiens.UCSC.hg19_1.3.16 MEDIPS_1.2 [3] gtools_2.6.2 BSgenome_1.16.3 [5] Biostrings_2.16.5 GenomicRanges_1.0.3 [7] IRanges_1.6.6 loaded via a namespace (and not attached): [1] Biobase_2.8.0 tools_2.11. Also, later on, when I call MEDIPS.plotCalibrationPlot, I get the following: > png("CalibrationPlot.png") > for(bar in calib_list){ #for each barcode: + counter=counter+1 + MEDIPS.plotCalibrationPlot(data=bar, linearFit=T, plot_chr="all", rpm=T, xrange=10, main=paste("Calibration plot (bar ", counter, ")", sep="")) + } Plotting calibration plot for all chromosomes. It is recommended to call a png() function before! *** caught segfault *** address 0x135d8f794d18, cause 'memory not mapped' Traceback: 1: axis(side = side, at = at, labels = labels, ...) 2: Axis.default(...) 3: Axis(...) 4: localAxis(if (is.null(y)) xy$x else x, side = 1, ...) 5: plot.default(signal, coupling, pch = ".", main = "Calibration plot", xlab = paste(descSignal, "", sep = ""), ylab = paste(seq_pattern, " coupling factor", sep = ""), col = "lightblue") 6: plot(signal, coupling, pch = ".", main = "Calibration plot", xlab = paste(descSignal, "", sep = ""), ylab = paste(seq_pattern, " coupling factor", sep = ""), col = "lightblue") 7: MEDIPS.plotCalibrationPlot(data = bar, linearFit = T, plot_chr = "all", rpm = T, xrange = 10, main = paste("Calibration plot (bar ", counter, ")", sep = "")) aborting ... Is this a problem on my side or is it in the code? Thank you in advance for your help! Best, Nora