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Sheena Scroggins
▴
30
@sheena-scroggins-4305
Last seen 10.2 years ago
Hello,
I am doing an meta analysis project with a group and we are having
issues
with POE and metaArray. poe.fit function is not found for any of us,
so we
are using poe.em.
library(simpleaffy)
library(metaArray)
library(Biobase)
library(MergeMaid)
data1 <- read.affy("treat1.txt")
eset1 = call.exprs(data1, "rma") #22283 features, 22 samples
cl1 = as.numeric(data1$Cancer == "Y") #Create class label
data1centered = t(scale(t(exprs(eset1)))) #Center and scale eset
data
data1.poe = poe.em(data1centered, cl1, threshold = 1e-05, every= 1000)
#perform POE transformation on centered eset data
####repeated for our 3 data sets, resulting in: data1.poe, data2.poe
and
data3.poe######
The next part is where we are stuck
common = intersect(rownames(data1.poe), rownames(data2.poe))
common = intersect(common, rownames(data3.poe))
summary(common)
Length Class Mode
0 NULL NULL
common
NULL
data1.poe = data1.poe[match(common, rownames(data1.poe)), ]
####Can't use
since 'common' is empty
Error in data1.poe[match(common, rownames(data1.poe)), ] :
incorrect number of dimensions
We are using the code from the metaArray vignette to guide us but are
stuck
on this part. Any help would be appreciated.
Sheena
> sessionInfo()
R version 2.11.1 (2010-05-31)
i386-pc-mingw32
locale:
[1] LC_COLLATE=English_United States.1252
[2] LC_CTYPE=English_United States.1252
[3] LC_MONETARY=English_United States.1252
[4] LC_NUMERIC=C
[5] LC_TIME=English_United States.1252
attached base packages:
[1] splines stats graphics grDevices utils datasets
methods
[8] base
other attached packages:
[1] hgu133acdf_2.6.0 MergeMaid_2.20.0 MASS_7.3-6
survival_2.35-8
[5] metaArray_1.24.0 simpleaffy_2.24.0 gcrma_2.20.0
genefilter_1.30.0
[9] affy_1.26.1 Biobase_2.8.0
loaded via a namespace (and not attached):
[1] affyio_1.16.0 annotate_1.26.1 AnnotationDbi_1.10.2
[4] Biostrings_2.16.9 DBI_0.2-5 IRanges_1.6.17
[7] preprocessCore_1.10.0 RSQLite_0.9-2 tools_2.11.1
[10] xtable_1.5-6
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