Dear Dr. Mattia Pelizzola£º I am sorry to trouble you! I am great interested in the methods in your paper named Genome-wide screen of promoter methylation identifies novel markers in melanoma. I have downloaded this paper from the linking as fellow: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720187/ The methods of identification of biomarkers is very good. But I have something wrong about it. *Identification of biomarkers* Intermediate and proximal promoter regions of each RefSeq were divided into six regions based on the distance from the TSS (-500 to -350, -200 to -50, -50 to +100, +100 to +250, and +250 to +500 bp, with respect to the TSS). The AMS for a given region was determined as the average of the AMS for the probes in that genomic area. I don't know you according to what rules to divide the RefSeq.Can you give me an example to descipe it? I will appreciate any help from you, thank you very much ! Best wishes Yours sincerely shujiema [[alternative HTML version deleted]]

Hi, Shujiema. This is an email list for discussing Bioconductor, so you might need to try a different place to get your answers. Sean On Mon, Sep 5, 2011 at 1:26 AM, ??? <msmashujie at="" gmail.com=""> wrote: > Dear Dr. Mattia Pelizzola? > > > > I am sorry to trouble you! > > > > I am great interested in the methods in your paper named Genome-wide screen > of promoter methylation identifies novel markers in melanoma. > > I have downloaded this paper from the linking as fellow: > > http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720187/ > > > > The methods of identification of biomarkers is very good. But I have > something wrong about it. > > > > *Identification of biomarkers* > > Intermediate and proximal promoter regions of each RefSeq were divided into > six regions based on the distance from the TSS (-500 to -350, -200 to -50, -50 > to +100, +100 to +250, and +250 to +500 bp, with respect to the TSS). The > AMS for a given region was determined as the average of the AMS for the > probes in that genomic area. > > > > I don't know you according to what rules to divide the RefSeq.Can you give > me an example to descipe it? > > > > I will appreciate any help from you, thank you very much ! > > Best wishes > > Yours sincerely > > ? ? ? ? ? ? ? ? ?shujiema > > ? ? ? ?[[alternative HTML version deleted]] > > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor >