I am using the limma application and am wondering whether there are is a QualityWeights function available for Imagene? If not, is there an alternative way to compute QualityWeights when using data from the Imagene image analysis program? Hope someone can help me out, Joyce van de Leemput ===============einde bericht======================== Dit bericht is verstuurd via http://www.twigger.nl. Overal ter wereld je bestaande mailadres bereikbaar. Stuur goedkoop SMS via http://www.twiggersms.nl

At 01:35 AM 17/03/2004, joycel_balaena.bio.vu.nl wrote: >I am using the limma application and am wondering whether there are is a >QualityWeights function available for Imagene? If not, is there an >alternative >way to compute QualityWeights when using data from the Imagene image analysis >program? We work with Imagene data very seldom and so have not developed our own weighting scheme for Imagene data. Since Image output comes with a 'Flag' column, the best I can suggest is that you can simply convert the flags to numeric weights, i.e., wt.fun=function(x) as.numeric(x$Flag==0) Does anyone who works with Imagene have a better suggestion? Gordon >Hope someone can help me out, >Joyce van de Leemput