Dear Liuyang, > wrong on getCdfInfo(object) : > > Could not obtain CDF environment, problems encountered: Specified > environment does not contain U133_X3Pa520049 Library - package > u133x3pa520049cdf not installed Bioconductor - u133x3pa520049cdf not > available > > Would you please tell me how to solve this problem? > I'm not an expert in this but maybe this helps somehow. That error means that you don't have annotation (CDF) package for your affymetrix chip. You can search here http://www.bioconductor.org/packages/release/data/annotation/ for the appropriate CDF package. Also, you can chack makecdfenv package. Best Wishes, Maciej Jo?czyk -- Maciej Jonczyk, Department of Plant Molecular Ecophysiology Faculty of Biology, University of Warsaw 02-096 Warsaw, Miecznikowa 1 Poland -- This email was Anti Virus checked by Astaro Security Gateway. http://www.astaro.com