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David
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860
@david-3335
Last seen 6.8 years ago
Hi,
I have done an RT-PCR experiment from which i have calculated fold
changes when comparing the expression of several genes in healthy vs
disease.
In a second independent experiment i have done the same so that i get
again a list of fold changes
healthy vs diseaseA (fold changes exp1) healthy vs diseaseA
(fold
changes exp2)
geneA 2 1.5
geneB 1.5 1.4
geneC 2.5 2.8
geneD 5 1.5
geneE 1.8 1.9
geneF 1.5 1.4
geneG 1.4 1.3
.....
For geneD I have 5 fold changes in one experiment and 1.5 fold
changes
in another expermient. Is there any stasticial method to see if the
difference between the two fold changes is significant for geneD ?
any
pvalue ?
thanks