hi i did two color array (agilent 44K two color) .one sample with two replicate. which test or algorithm can i use to find differentially regulated genes in R package. i have one samples with two techincal replicate: 1.normal = cy3 treated= Cy5 = Cy5/Cy3 2.normal= Cy3 treated = Cy5 = Cy5/Cy3 thanx neeraj [[alternative HTML version deleted]]