Entering edit mode
Magdalena Wozniak
▴
50
@magdalena-wozniak-5004
Last seen 10.6 years ago
Hi,
I am trying to upload TCGA level1 methylation data from 27k. I've
tried to
follow instructions for Glioblastoma example as well but I had a
problem
with installation of TCGAGBM package so I was not able see the format
of
the files of the example data. I've tried different versions of the
following script but at some point (I think from the point I
indicated) it
does not work properly. I would be very grateful for help.
Best wishes,
Magdalena
homedir="D:/TCGA Methylation Analysis/DATA/KIRC/LEVEL
1/Pairs_HumanMethylation27"
dirs=list.files(homedir)
batchNames=gsub("jhu-usc.edu_KIRC.HumanMethylation27.","",dirs)
tmp=sapply(strsplit(batchNames,"\."),function(x) as.numeric(x[1:2]))
files=vector("list",length(dirs))
ngenes=27578
for(i in seq(along=dirs)){
tmpPath=file.path(homedir,dirs[i])
fns=list.files(tmpPath)
files[[i]]=fns[grep("lvl-1",fns)]
}
nfiles=sapply(files,length)
starts=c(0,cumsum(nfiles[-length(nfiles)]))
*
### FROM HERE SOMETHING DOESN'T WORK*
# b = tmp$Methylated_Signal_Intensity..M./
(tmp$Methylated_Signal_Intensity..M.
+ tmp$Un.Methylated_Signal_Intensity..U.+100)
# betas=matrix(b,nrow=ngenes,ncol=sum(nfiles))
betas=matrix(NA,nrow=ngenes,ncol=sum(nfiles))
cnames=vector("character",sum(nfiles))
batch=factor(rep(seq(along=files),nfiles))
for(i in seq(along=dirs)){
cat(i)
tmpPath=file.path(homedir,dirs[i])
fns=files[[i]]
for(j in seq(along=fns)){
cat(".")
###Get the sample name
tmp=strsplit(readLines(file.path(tmpPath,fns[j]),n=1),"\t")
cnames[ j+starts[i] ]=tmp[[1]][2]
##Get the Beta values
tmp=read.delim(file.path(tmpPath,fns[j]),skip=1,check.names=FALSE,as.i
s
=TRUE)
b=
betaIndex=grep("[bB]eta",names(tmp)) ##the column name change!!
betas[,j+starts[i]]=tmp[,betaIndex]
###Check "gene" names in same order
if(i==1 & j==1) gnames=tmp[,"CompositeElement REF"] else
if(!identical(gnames,tmp[,"CompositeElement REF"])) stop("Genes not in
order")
}
cat("\n")
}
colnames(betas)=cnames
rownames(betas)=gnames
save(betas,batch,batchNames,file="betas.rda")
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