Hi all, I'd like to use the qspline normalization method: normalize.celfile.qspline {affy} I have two problems with this: a) I would like to use this normalization on cDNA data. Am I correct in interpreting that this method can either be fun directly on a Cel.container object, or alternately can be called a data.matrix of intensity values and a target vector? Or do I need to do something in addition to use it for non-Affy data? b) I am a bit unclear on some of the arguments, and I am unsure how to pick appropriate levels. In particular, I am unclear about how to determine realistic values for: fit.iters spline.method spar incl.ends na.rm Any advice or ideas would be very much appreciated. And if this isn't the right place for these questions, guidance on where to go would also be welcome. Paul