Hi, I am having gsnnormalize error during pval calculation. I am pasting my eset below, could you please advise me if there is problem with the "eset" and if there is do i need to subset the phenodata to get some sort of solution. Thanks M Zaka Phd Student of Bioinformatics University of Bradford, UK pVals = gsealmPerm(eset, ~disease.state, mSigDB, nperm = 1000) Error in GSNormalize(obsRaw$tstat[2, ], incidence = mat, ...) : GSNormalize: non-conforming matrices ExpressionSet (storageMode: lockedEnvironment) assayData: 22283 features, 36 samples element names: exprs protocolData: none phenoData sampleNames: GSM512539 GSM512540 ... GSM512565 (36 total) varLabels: sample specimen disease.state description varMetadata: labelDescription featureData featureNames: 1007_s_at 1053_at ... AFFX-TrpnX-M_at (22283 total) fvarLabels: ID Gene title ... GO:Component ID (21 total) fvarMetadata: Column labelDescription experimentData: use 'experimentData(object)' pubMedIds: 20197764 Annotation: