Problems with section 3.4 in vignette GenomicRangesUseCases.pdf
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Yue Li ▴ 370
@yue-li-5245
Last seen 8.9 years ago
USA
Dear List, This is a question that has been asked before in the message titled "GenomicRanges Use Cases - subsetByOverlaps". But I just want to confirm that there is a mistake in section 3.4 of the vignette GenomicRangesUseCases.pdf. Basically, it was claimed in the section to look for "ranges that did not overlap with any of the known exons from Saccharomycess cerevisiae." using: filtData <- subsetByOverlaps(aligns, exonRanges) However, filtData in fact contains exactly the read regions that DO overlap with the known exons. To get ""ranges that did not overlap with any of the known exons", it's been suggested in that thread to instead use: not.exons <- aligns[!aligns %in% exonRanges] Is the latter in fact the CORRECT way to do it or the way in the vignette? Thanks in advance! Yue [[alternative HTML version deleted]]
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@valerie-obenchain-4275
Last seen 2.9 years ago
United States
Hi Yue, Yes, the vignette was incorrect. It has been fixed in version 1.8.4 in release and 1.9.9 in the devel branch. These versions should be available in ~24 hours through biocLite() or now from the svn repositories. Thanks for pointing out this bug and sorry we missed the previous discussion of the topic. We would suggest using not.exons <- aligns[!aligns %in% exonRanges] as posted in the last thread. Valerie The vignette has been updated in release devel 1.9.9 On 04/24/2012 06:52 PM, Yue Li wrote: > Dear List, > > This is a question that has been asked before in the message titled "GenomicRanges Use Cases - subsetByOverlaps". But I just want to confirm that there is a mistake in section 3.4 of the vignette GenomicRangesUseCases.pdf. > > Basically, it was claimed in the section to look for "ranges that did not overlap with any of the known exons from Saccharomycess cerevisiae." using: > > filtData<- subsetByOverlaps(aligns, exonRanges) > > However, filtData in fact contains exactly the read regions that DO overlap with the known exons. > > To get ""ranges that did not overlap with any of the known exons", it's been suggested in that thread to instead use: > > not.exons<- aligns[!aligns %in% exonRanges] > > Is the latter in fact the CORRECT way to do it or the way in the vignette? > > Thanks in advance! > Yue > > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
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Thanks Valerie. Yue On 2012-04-26, at 2:58 PM, Valerie Obenchain wrote: > Hi Yue, > > Yes, the vignette was incorrect. It has been fixed in version 1.8.4 in release and 1.9.9 in the devel branch. These versions should be available in ~24 hours through biocLite() or now from the svn repositories. Thanks for pointing out this bug and sorry we missed the previous discussion of the topic. > > We would suggest using > > not.exons <- aligns[!aligns %in% exonRanges] > > as posted in the last thread. > > Valerie > > > > > > The vignette has been updated in release devel 1.9.9 > > > On 04/24/2012 06:52 PM, Yue Li wrote: >> Dear List, >> >> This is a question that has been asked before in the message titled "GenomicRanges Use Cases - subsetByOverlaps". But I just want to confirm that there is a mistake in section 3.4 of the vignette GenomicRangesUseCases.pdf. >> >> Basically, it was claimed in the section to look for "ranges that did not overlap with any of the known exons from Saccharomycess cerevisiae." using: >> >> filtData<- subsetByOverlaps(aligns, exonRanges) >> >> However, filtData in fact contains exactly the read regions that DO overlap with the known exons. >> >> To get ""ranges that did not overlap with any of the known exons", it's been suggested in that thread to instead use: >> >> not.exons<- aligns[!aligns %in% exonRanges] >> >> Is the latter in fact the CORRECT way to do it or the way in the vignette? >> >> Thanks in advance! >> Yue >> >> >> [[alternative HTML version deleted]] >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at r-project.org >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor >
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