Entering edit mode
Dear List,
This is a question that has been asked before in the message titled
"GenomicRanges Use Cases - subsetByOverlaps". But I just want to
confirm that there is a mistake in section 3.4 of the vignette
GenomicRangesUseCases.pdf.
Basically, it was claimed in the section to look for "ranges that did
not overlap with any of the known exons from Saccharomycess
cerevisiae." using:
filtData <- subsetByOverlaps(aligns, exonRanges)
However, filtData in fact contains exactly the read regions that DO
overlap with the known exons.
To get ""ranges that did not overlap with any of the known exons",
it's been suggested in that thread to instead use:
not.exons <- aligns[!aligns %in% exonRanges]
Is the latter in fact the CORRECT way to do it or the way in the
vignette?
Thanks in advance!
Yue
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