GOSim yeast
2
0
Entering edit mode
Guest User ★ 13k
@guest-user-4897
Last seen 10.3 years ago
Hello, I'm trying to use GOSim with yeast but with no luck. The function "setEvidenceLevel(organism="yeast")" doesn't seem to work. Using the org.Sc.sgd.db library, I obtain a long list of GO terms for "856452" Yet, getGOInfo("856452") yields, No GO information available for these genes! Any help would be greatly appreciated. Yours, Fred Hoffman -- output of sessionInfo(): ID = "YHR055C" > > # get Entrez Gene ID > get(ID, org.Sc.sgdENTREZID) [1] "856452" > # get GO terms > get(ID, org.Sc.sgdGO) $`GO:0010273` $`GO:0010273`$GOID [1] "GO:0010273" $`GO:0010273`$Evidence [1] "IMP" $`GO:0010273`$Ontology [1] "BP" . . . >setEvidenceLevel(organism="yeast") > getGOInfo("856452") Error in getGOInfo("856452") : No GO information available for these genes! > setEvidenceLevel(organism="org.Sc.sgd.db") [1] "-> retrieving GO information for all available genes for organism 'org.Sc.sgd.db' in GO database" [1] "-> filtering GO terms according to evidence levels 'all'" > getGOInfo("856452") Error in getGOInfo("856452") : No GO information available for these genes! -- Sent via the guest posting facility at bioconductor.org.
GO Yeast GOSim GO Yeast GOSim • 2.2k views
ADD COMMENT
0
Entering edit mode
Marc Carlson ★ 7.2k
@marc-carlson-2264
Last seen 8.4 years ago
United States
Hi Fred, I can't speak to what is happening with GOSim:::getGOInfo. But if you are using the most recent version of bioconductor, then you could also do this kind of lookup like this: library(org.Sc.sgd.db) ID = "YHR055C" select(org.Sc.sgd.db, keys=ID, cols = c("GO","ENTREZID"), keytype="ORF") Marc On 05/22/2012 05:31 AM, Fred Hoffman [guest] wrote: > Hello, > > I'm trying to use GOSim with yeast but with no luck. The function > "setEvidenceLevel(organism="yeast")" doesn't seem to work. > > Using the org.Sc.sgd.db library, I obtain a long list of GO terms for "856452" > > Yet, getGOInfo("856452") yields, > > No GO information available for these genes! > > Any help would be greatly appreciated. > > Yours, > > Fred Hoffman > > -- output of sessionInfo(): > > > ID = "YHR055C" >> # get Entrez Gene ID >> get(ID, org.Sc.sgdENTREZID) > [1] "856452" > >> # get GO terms >> get(ID, org.Sc.sgdGO) > $`GO:0010273` > $`GO:0010273`$GOID > [1] "GO:0010273" > > $`GO:0010273`$Evidence > [1] "IMP" > > $`GO:0010273`$Ontology > [1] "BP" > > . > . > . >> setEvidenceLevel(organism="yeast") >> getGOInfo("856452") > Error in getGOInfo("856452") : > No GO information available for these genes! >> setEvidenceLevel(organism="org.Sc.sgd.db") > [1] "-> retrieving GO information for all available genes for organism 'org.Sc.sgd.db' in GO database" > [1] "-> filtering GO terms according to evidence levels 'all'" >> getGOInfo("856452") > Error in getGOInfo("856452") : > No GO information available for these genes! > > > -- > Sent via the guest posting facility at bioconductor.org. > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
ADD COMMENT
0
Entering edit mode
solis • 0
@solis-12578
Last seen 7.8 years ago

Follow the next commands:

library(org.Sc.sgd.db)

setEvidenceLevel(evidences="all", organism=org.Sc.sgdORGANISM, gomap=org.Sc.sgdGO)

setOntology("BP", loadIC=FALSE)

calcICs(DIR="~/R/x86_64-redhat-linux-gnu-library/3.3/GOSim/data") # change DIR accordingly otherwise is save in the current directory

# Upload the ICs files

setOntology("BP", DIR="~/R/x86_64-redhat-linux-gnu-library/3.3/GOSim/data")

The gene IDs will depend on the database that you used when calling setEvidenceLevel. For the org.Sc.sgdGO, this is the systematic names. One way to know is by typing:

head(get("gomap", envir=GOSimEnv))

So to get the GO information or Gene Similarity use systematic IDs. For example:

getGOInfo(c("YHR043C"))
getGeneSim(c("HRA1", "ICR1"))

ADD COMMENT

Login before adding your answer.

Traffic: 400 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6