I've read the users guide but I'm not clear whether limma is able to calculate paired t statistics? e.g. Person A, B, C, at time 0, and 1 hour after treatment-- in which I am interested in the treatment effect. Using affy and rma values. Stephen ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager (it.support@wibr.ucl.ac.uk). All files are scanned for viruses.