how to set parameter of DESeq function estimateDispersions i have 6 groups data and each group contains 3 samples conditions=c(rep("DL7", 3),rep("DL27", 3),rep("DLCK", 3),rep("L27", 3),rep("L7", 3),rep("LCK", 3)) i will compare them between two groups. should i use cds <- estimateDispersions(cds, method = "per-condition",sharingMode="fit-only") ? what difference between using per-condition and blind? why say blind lead to loss of power; -- shan gao Room 231(Dr.Fei lab) Boyce Thompson Institute Cornell University Tower Road, Ithaca, NY 14853-1801 Office phone: 1-607-254-1267(day) Official email:sg839 at cornell.edu Facebook:http://www.facebook.com/profile.php?id=100001986532253