LIMMA: question about design and contrasts
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@patrick-de-boever-5561
Last seen 10.5 years ago
Dear all, We have used single color arrays for the following experiment: 25 individuals were split in 2 groups: location 1 and location 2. All individuals underwent an intervention. The individuals were analyzed twice: once before intervention and once after intervention. Which design should we use within LIMMA for assessing the effect of the location, the effect of the intervention, and possible interaction effect? Thank you for your kind reaction, Patrick Patrick De Boever, PhD, MSc Environment and Health Research Centre for Environmental Sciences VITO NV Hasselt University - Campus Diepenbeek Industriezone Vlasmeer 7, 2400 Mol, Belgium Agoralaan building D, 3590, Diepenbeek, Belgium Tel. + 32 14 33 51 45 patrick.deboever@uhasselt.be Fax. + 32 14 58 05 23 patrick.deboever@vito.be Visit our website: www.vito.be/english Sent from my iPad [[alternative HTML version deleted]]
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@james-w-macdonald-5106
Last seen 1 day ago
United States
Hi Patrick, On 10/19/2012 1:10 AM, Patrick De Boever wrote: > Dear all, > > We have used single color arrays for the following experiment: > > 25 individuals were split in 2 groups: location 1 and location 2. All individuals underwent an intervention. > The individuals were analyzed twice: once before intervention and once after intervention. > > Which design should we use within LIMMA for assessing the effect of the location, the effect of the intervention, and possible interaction effect? I think you will need to give more information. What do you mean exactly by '25 individuals were split in 2 groups: location 1 and location 2.' ? That can be interpreted several ways. Are the locations sampling locations (e.g., one group was sampled from location 1 on their body, and the other was sampled from location 2) or are these physical locations (one group was recruited and samples were taken by lab 1 and the other group went to lab 2, and the two labs are in different parts of the country/university/world/whatever). Best, Jim > > Thank you for your kind reaction, > > Patrick > > > > Patrick De Boever, PhD, MSc > > Environment and Health Research Centre for Environmental Sciences > VITO NV Hasselt University - Campus Diepenbeek > Industriezone Vlasmeer 7, 2400 Mol, Belgium Agoralaan building D, 3590, Diepenbeek, Belgium > Tel. + 32 14 33 51 45 patrick.deboever at uhasselt.be > Fax. + 32 14 58 05 23 > patrick.deboever at vito.be > > Visit our website: www.vito.be/english > > > > Sent from my iPad > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician University of Washington Environmental and Occupational Health Sciences 4225 Roosevelt Way NE, # 100 Seattle WA 98105-6099
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