rs ids Retrieval using biomaRt
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Voke AO ▴ 760
@voke-ao-4830
Last seen 10.3 years ago
Hi all, I've been trying to retrieve SNP rsids using biomaRt for a number of SNPs where I have their positions. My input file looks like this: 1 888899000 888899000 where, 1 = chromosome number and the second and third numbers are the position of the SNP. My code looks like this: library(biomaRt) mart2 = useMart(biomart="snp", dataset="hsapiens_snp") AFRASN=read.delim("AFR_ASN_neg8P_no_rsids.txt", header = TRUE) results = getBM(attributes = c("refsnp_id", "allele","chr_name", "chrom_strand","consequence_type_tv"), filters = "chromosomal_region", values = AFRASN$CHR, mart = mart2) I must be doing something wrong because it's not working for me. Any suggestions will be greatly appreciated. > sessionInfo() R version 2.15.0 (2012-03-30) Platform: i386-pc-mingw32/i386 (32-bit) locale: [1] LC_COLLATE=English_.1252 LC_CTYPE=English_.1252 [3] LC_MONETARY=English_.1252 LC_NUMERIC=C [5] LC_TIME=English_.1252 attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] biomaRt_2.12.0 loaded via a namespace (and not attached): [1] RCurl_1.91-1.1 XML_3.9-4.1 > Regards, Avoks
SNP biomaRt SNP biomaRt • 3.7k views
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@vincent-j-carey-jr-4
Last seen 3 months ago
United States
On Sat, Nov 3, 2012 at 6:15 AM, Ovokeraye Achinike-Oduaran < ovokeraye@gmail.com> wrote: > Hi all, > > I've been trying to retrieve SNP rsids using biomaRt for a number of > SNPs where I have their positions. My input file looks like this: > > 1 888899000 888899000 > > where, > 1 = chromosome number and the second and third numbers are the > position of the SNP. > > My code looks like this: > > library(biomaRt) > mart2 = useMart(biomart="snp", dataset="hsapiens_snp") > AFRASN=read.delim("AFR_ASN_neg8P_no_rsids.txt", header = TRUE) > results = getBM(attributes = c("refsnp_id", "allele","chr_name", > "chrom_strand","consequence_type_tv"), > filters = "chromosomal_region", values = AFRASN$CHR, mart = mart2) > > I must be doing something wrong because it's not working for me. Any > suggestions will be greatly appreciated. > > your filter/value spec does not seem valid. i do not know how to determine validity. but the following yields 23 records results = getBM(attributes = c("refsnp_id", "allele","chr_name", "chrom_strand","consequence_type_tv"), filters = c("chr_name", "chrom_start", "chrom_end"), values=list("20", 100000, 101000), mart=mart2) > > sessionInfo() > R version 2.15.0 (2012-03-30) > Platform: i386-pc-mingw32/i386 (32-bit) > > locale: > [1] LC_COLLATE=English_.1252 LC_CTYPE=English_.1252 > [3] LC_MONETARY=English_.1252 LC_NUMERIC=C > [5] LC_TIME=English_.1252 > > attached base packages: > [1] stats graphics grDevices utils datasets methods base > > other attached packages: > [1] biomaRt_2.12.0 > > loaded via a namespace (and not attached): > [1] RCurl_1.91-1.1 XML_3.9-4.1 > > > > Regards, > > Avoks > > _______________________________________________ > Bioconductor mailing list > Bioconductor@r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > [[alternative HTML version deleted]]
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Thanks Vincent. You are right about the filters. One more thing: how would this work with a batch input "values" list i.e reading the positions from a file? For example: CHR 1, 11182261, 11182261 1, 11264032, 11264032 1, 11264036, 11264036 1, 11282246, 11282246 1, 11292945, 11292945 1, 11306660, 11306660 1, 11314655, 11314655 Using the same code but specifying values = AFRASN$CHR gives me an error --"Error in getBM(attributes = c("refsnp_id", "allele", "chr_name", "chrom_strand", : If using multiple filters, the 'value' has to be a list...." Thanks again. Regards, Avoks On Sat, Nov 3, 2012 at 1:46 PM, Vincent Carey <stvjc at="" channing.harvard.edu=""> wrote: > > > On Sat, Nov 3, 2012 at 6:15 AM, Ovokeraye Achinike-Oduaran > <ovokeraye at="" gmail.com=""> wrote: >> >> Hi all, >> >> I've been trying to retrieve SNP rsids using biomaRt for a number of >> SNPs where I have their positions. My input file looks like this: >> >> 1 888899000 888899000 >> >> where, >> 1 = chromosome number and the second and third numbers are the >> position of the SNP. >> >> My code looks like this: >> >> library(biomaRt) >> mart2 = useMart(biomart="snp", dataset="hsapiens_snp") >> AFRASN=read.delim("AFR_ASN_neg8P_no_rsids.txt", header = TRUE) >> results = getBM(attributes = c("refsnp_id", "allele","chr_name", >> "chrom_strand","consequence_type_tv"), >> filters = "chromosomal_region", values = AFRASN$CHR, mart = mart2) >> >> I must be doing something wrong because it's not working for me. Any >> suggestions will be greatly appreciated. >> > > your filter/value spec does not seem valid. i do not know how to determine > validity. but > the following yields 23 records > > results = getBM(attributes = c("refsnp_id", "allele","chr_name", > "chrom_strand","consequence_type_tv"), > filters = c("chr_name", "chrom_start", "chrom_end"), > values=list("20", > 100000, 101000), mart=mart2) > > >> >> > sessionInfo() >> R version 2.15.0 (2012-03-30) >> Platform: i386-pc-mingw32/i386 (32-bit) >> >> locale: >> [1] LC_COLLATE=English_.1252 LC_CTYPE=English_.1252 >> [3] LC_MONETARY=English_.1252 LC_NUMERIC=C >> [5] LC_TIME=English_.1252 >> >> attached base packages: >> [1] stats graphics grDevices utils datasets methods base >> >> other attached packages: >> [1] biomaRt_2.12.0 >> >> loaded via a namespace (and not attached): >> [1] RCurl_1.91-1.1 XML_3.9-4.1 >> > >> >> Regards, >> >> Avoks >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at r-project.org >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor > >
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arrange for the start and end coordinates to be numerical vectors. how you do this in R depends on your file structure and your input call; if problematic, please query r-help. once you have acquired the vectors, you can follow the prescriptions of the man page. For example results = getBM(attributes = c("refsnp_id", "allele","chr_name", "chrom_strand","consequence_type_tv", "chrom_start"), filters = c("chr_name", "chrom_start", "chrom_end"), values=list("20", c(100066, 100134), c(100066, 100134)), mart=mart2) # replace the two calls to c() here with # your vector(s) returns two records On Sat, Nov 3, 2012 at 8:38 AM, Ovokeraye Achinike-Oduaran < ovokeraye@gmail.com> wrote: > Thanks Vincent. You are right about the filters. One more thing: how > would this work with a batch input "values" list i.e reading the > positions from a file? For example: > > CHR > 1, 11182261, 11182261 > 1, 11264032, 11264032 > 1, 11264036, 11264036 > 1, 11282246, 11282246 > 1, 11292945, 11292945 > 1, 11306660, 11306660 > 1, 11314655, 11314655 > > Using the same code but specifying values = AFRASN$CHR gives me an > error --"Error in getBM(attributes = c("refsnp_id", "allele", > "chr_name", "chrom_strand", : If using multiple filters, the > 'value' has to be a list...." > > Thanks again. > > Regards, > > Avoks > On Sat, Nov 3, 2012 at 1:46 PM, Vincent Carey > <stvjc@channing.harvard.edu> wrote: > > > > > > On Sat, Nov 3, 2012 at 6:15 AM, Ovokeraye Achinike-Oduaran > > <ovokeraye@gmail.com> wrote: > >> > >> Hi all, > >> > >> I've been trying to retrieve SNP rsids using biomaRt for a number of > >> SNPs where I have their positions. My input file looks like this: > >> > >> 1 888899000 888899000 > >> > >> where, > >> 1 = chromosome number and the second and third numbers are the > >> position of the SNP. > >> > >> My code looks like this: > >> > >> library(biomaRt) > >> mart2 = useMart(biomart="snp", dataset="hsapiens_snp") > >> AFRASN=read.delim("AFR_ASN_neg8P_no_rsids.txt", header = TRUE) > >> results = getBM(attributes = c("refsnp_id", "allele","chr_name", > >> "chrom_strand","consequence_type_tv"), > >> filters = "chromosomal_region", values = AFRASN$CHR, mart = > mart2) > >> > >> I must be doing something wrong because it's not working for me. Any > >> suggestions will be greatly appreciated. > >> > > > > your filter/value spec does not seem valid. i do not know how to > determine > > validity. but > > the following yields 23 records > > > > results = getBM(attributes = c("refsnp_id", "allele","chr_name", > > "chrom_strand","consequence_type_tv"), > > filters = c("chr_name", "chrom_start", "chrom_end"), > > values=list("20", > > 100000, 101000), mart=mart2) > > > > > >> > >> > sessionInfo() > >> R version 2.15.0 (2012-03-30) > >> Platform: i386-pc-mingw32/i386 (32-bit) > >> > >> locale: > >> [1] LC_COLLATE=English_.1252 LC_CTYPE=English_.1252 > >> [3] LC_MONETARY=English_.1252 LC_NUMERIC=C > >> [5] LC_TIME=English_.1252 > >> > >> attached base packages: > >> [1] stats graphics grDevices utils datasets methods base > >> > >> other attached packages: > >> [1] biomaRt_2.12.0 > >> > >> loaded via a namespace (and not attached): > >> [1] RCurl_1.91-1.1 XML_3.9-4.1 > >> > > >> > >> Regards, > >> > >> Avoks > >> > >> _______________________________________________ > >> Bioconductor mailing list > >> Bioconductor@r-project.org > >> https://stat.ethz.ch/mailman/listinfo/bioconductor > >> Search the archives: > >> http://news.gmane.org/gmane.science.biology.informatics.conductor > > > > > [[alternative HTML version deleted]]
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Thanks Vincent. The original code actually was alright. The problem was with my input file...it had to be colon (:)-separated. Thanks again. Regards, Avoks On Sat, Nov 3, 2012 at 3:03 PM, Vincent Carey <stvjc at="" channing.harvard.edu=""> wrote: > arrange for the start and end coordinates to be numerical vectors. how you > do this in R depends on your file structure and your input call; if > problematic, please query r-help. once you have acquired the vectors, you > can follow the prescriptions of the man page. For example > > results = getBM(attributes = c("refsnp_id", "allele","chr_name", > "chrom_strand","consequence_type_tv", "chrom_start"), > filters = c("chr_name", "chrom_start", "chrom_end"), > values=list("20", > c(100066, 100134), c(100066, 100134)), mart=mart2) # replace the two > calls to c() here with > # your vector(s) > > returns two records > > > On Sat, Nov 3, 2012 at 8:38 AM, Ovokeraye Achinike-Oduaran > <ovokeraye at="" gmail.com=""> wrote: >> >> Thanks Vincent. You are right about the filters. One more thing: how >> would this work with a batch input "values" list i.e reading the >> positions from a file? For example: >> >> CHR >> 1, 11182261, 11182261 >> 1, 11264032, 11264032 >> 1, 11264036, 11264036 >> 1, 11282246, 11282246 >> 1, 11292945, 11292945 >> 1, 11306660, 11306660 >> 1, 11314655, 11314655 >> >> Using the same code but specifying values = AFRASN$CHR gives me an >> error --"Error in getBM(attributes = c("refsnp_id", "allele", >> "chr_name", "chrom_strand", : If using multiple filters, the >> 'value' has to be a list...." >> >> Thanks again. >> >> Regards, >> >> Avoks >> On Sat, Nov 3, 2012 at 1:46 PM, Vincent Carey >> <stvjc at="" channing.harvard.edu=""> wrote: >> > >> > >> > On Sat, Nov 3, 2012 at 6:15 AM, Ovokeraye Achinike-Oduaran >> > <ovokeraye at="" gmail.com=""> wrote: >> >> >> >> Hi all, >> >> >> >> I've been trying to retrieve SNP rsids using biomaRt for a number of >> >> SNPs where I have their positions. My input file looks like this: >> >> >> >> 1 888899000 888899000 >> >> >> >> where, >> >> 1 = chromosome number and the second and third numbers are the >> >> position of the SNP. >> >> >> >> My code looks like this: >> >> >> >> library(biomaRt) >> >> mart2 = useMart(biomart="snp", dataset="hsapiens_snp") >> >> AFRASN=read.delim("AFR_ASN_neg8P_no_rsids.txt", header = TRUE) >> >> results = getBM(attributes = c("refsnp_id", "allele","chr_name", >> >> "chrom_strand","consequence_type_tv"), >> >> filters = "chromosomal_region", values = AFRASN$CHR, mart = >> >> mart2) >> >> >> >> I must be doing something wrong because it's not working for me. Any >> >> suggestions will be greatly appreciated. >> >> >> > >> > your filter/value spec does not seem valid. i do not know how to >> > determine >> > validity. but >> > the following yields 23 records >> > >> > results = getBM(attributes = c("refsnp_id", "allele","chr_name", >> > "chrom_strand","consequence_type_tv"), >> > filters = c("chr_name", "chrom_start", "chrom_end"), >> > values=list("20", >> > 100000, 101000), mart=mart2) >> > >> > >> >> >> >> > sessionInfo() >> >> R version 2.15.0 (2012-03-30) >> >> Platform: i386-pc-mingw32/i386 (32-bit) >> >> >> >> locale: >> >> [1] LC_COLLATE=English_.1252 LC_CTYPE=English_.1252 >> >> [3] LC_MONETARY=English_.1252 LC_NUMERIC=C >> >> [5] LC_TIME=English_.1252 >> >> >> >> attached base packages: >> >> [1] stats graphics grDevices utils datasets methods base >> >> >> >> other attached packages: >> >> [1] biomaRt_2.12.0 >> >> >> >> loaded via a namespace (and not attached): >> >> [1] RCurl_1.91-1.1 XML_3.9-4.1 >> >> > >> >> >> >> Regards, >> >> >> >> Avoks >> >> >> >> _______________________________________________ >> >> Bioconductor mailing list >> >> Bioconductor at r-project.org >> >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> >> Search the archives: >> >> http://news.gmane.org/gmane.science.biology.informatics.conductor >> > >> > > >
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