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Vada Wilcox
▴
10
@vada-wilcox-795
Last seen 10.2 years ago
Dear all,
I have been using RMA succesfully for a while now, but in the past I
have
only used it on a small amount of slides. I would like to do my study
on a
larger scale now, with data (series of experiments) from other
researchers
as well. My questions is the following: if I want to study, let's say
200
slides, do I have to read them all into R at once (so together I mean,
with
read.affy() in package affy), or is it OK to read them series by
series (so
all wild types and controls of one researcher at a time)?
If it is really necessary to read all of them in at one time how much
RAM
would I need (for let's say 200 CELfiles) and how can I raise the RAM?
I now
it's possible to raise it by using 'max vsize = ...' but I haven't
been able
to do it succesfully for 200 experiments though. Can somebody help me
on
this?
Many thanks in advance,
Vada
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