Hi there, I wonder if anyone have compared the data correlation between the same sample labeled by Affy's one cycle labeling and two cycle labeling kit? The amount of some of my pooled samples is in the lower end of the one cycle labeling kit. Life would be easier if I use the two cycle kit. But as we all know, more processing steps and amplication may lead to variation. I am not sure whether I want to use the two cycle kit or just collect more samples. Any thoughts? Regards, Fai -- Yuk Fai Leung Department of Molecular and Cellular Biology Harvard University BL 2079, 16 Divinity Avenue Cambridge, MA 02138 Tel: 617-495-2599 Fax: 617-496-3321 email: yfleung@mcb.harvard.edu; yfleung@genomicshome.com URL: http://genomicshome.com

Dear Fai, I you have homogenous datasets with either only 1x amplified samples or only 2x amplified samples, that's ok. If you mix them, you will not be able to compare them. They will form separate clusters. Best regards, Michael On Fri, 11 Jun 2004, YUK FAI LEUNG wrote: > Hi there, > > I wonder if anyone have compared the data correlation between the same > sample labeled by Affy's one cycle labeling and two cycle labeling kit? > The amount of some of my pooled samples is in the lower end of the one > cycle labeling kit. Life would be easier if I use the two cycle kit. But > as we all know, more processing steps and amplication may lead to > variation. I am not sure whether I want to use the two cycle kit or just > collect more samples. Any thoughts? > > Regards, > Fai