topGO quick start guide error
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Fix Ace ▴ 100
@fix-ace-5689
Last seen 9.7 years ago
United States
I just installed topGO and tried to run their example: when I typed:     affyLib <- paste(annotation(ALL), "db", sep = ".")     affyLib     [1] "hgu95av2.db"     library(package = affyLib, character.only = TRUE) got error message:     Error in library(package = affyLib, character.only = TRUE) :   there is no package called ‘hgu95av2.db’ Anybody has idea what is going on here? Thanks:) [[alternative HTML version deleted]]
topGO topGO • 3.9k views
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@james-w-macdonald-5106
Last seen 2 days ago
United States
On 1/8/2013 5:31 PM, Fix Ace wrote: > I just installed topGO and tried to run their example: > > when I typed:? > ? ? ? affyLib<- paste(annotation(ALL), "db", sep = ".") > ? ? ? affyLib > ? ? ? [1] "hgu95av2.db" > ? ? ? ? library(package = affyLib, character.only = TRUE) > > got error message: > ? ? ? Error in library(package = affyLib, character.only = TRUE) : > ? there is no package called ???hgu95av2.db??? That error message tells you what is wrong - you don't have the hgu95av2.db package installed. Best, Jim > > > Anybody has idea what is going on here? Thanks:) > [[alternative HTML version deleted]] > > > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician University of Washington Environmental and Occupational Health Sciences 4225 Roosevelt Way NE, # 100 Seattle WA 98105-6099
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Thank you very much for your reply. Does it mean I will have to run: bioclite("hgu95av2.db") I am really new to bioconductor, not quite sure how it runs... ________________________________ From: James W. MacDonald <jmacdon@uw.edu> Cc: "bioconductor@r-project.org" <bioconductor@r-project.org> Sent: Wednesday, January 9, 2013 7:58 AM Subject: Re: [BioC] topGO quick start guide error On 1/8/2013 5:31 PM, Fix Ace wrote: > I just installed topGO and tried to run their example: > > when I typed: >     affyLib<- paste(annotation(ALL), "db", sep = ".") >     affyLib >     [1] "hgu95av2.db" >     library(package = affyLib, character.only = TRUE) > > got error message: >     Error in library(package = affyLib, character.only = TRUE) : >   there is no package called ‘hgu95av2.db’ That error message tells you what is wrong - you don't have the hgu95av2.db package installed. Best, Jim > > > Anybody has idea what is going on here? Thanks:) >     [[alternative HTML version deleted]] > > > > _______________________________________________ > Bioconductor mailing list > Bioconductor@r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician University of Washington Environmental and Occupational Health Sciences 4225 Roosevelt Way NE, # 100 Seattle WA 98105-6099 [[alternative HTML version deleted]]
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On Wed, Jan 9, 2013 at 6:36 PM, Fix Ace <acefix at="" rocketmail.com=""> wrote: > > Thank you very much for your reply. > > Does it mean I will have to run: > > bioclite("hgu95av2.db") Yes. If that doesn't work, do this first: source("http://bioconductor.org/biocLite.R") Dan > > I am really new to bioconductor, not quite sure how it runs... > > > ________________________________ > From: James W. MacDonald <jmacdon at="" uw.edu=""> > > Cc: "bioconductor at r-project.org" <bioconductor at="" r-project.org=""> > Sent: Wednesday, January 9, 2013 7:58 AM > Subject: Re: [BioC] topGO quick start guide error > > > > On 1/8/2013 5:31 PM, Fix Ace wrote: >> I just installed topGO and tried to run their example: >> >> when I typed:? >> ? ? ? affyLib<- paste(annotation(ALL), "db", sep = ".") >> ? ? ? affyLib >> ? ? ? [1] "hgu95av2.db" >> ? ? ? ? library(package = affyLib, character.only = TRUE) >> >> got error message: >> ? ? ? Error in library(package = affyLib, character.only = TRUE) : >> ? there is no package called ???hgu95av2.db??? > > That error message tells you what is wrong - you don't have the > hgu95av2.db package installed. > > Best, > > Jim > > >> >> >> Anybody has idea what is going on here? Thanks:) >> [[alternative HTML version deleted]] >> >> >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at r-project.org >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > > -- > James W. MacDonald, M.S. > Biostatistician > University of Washington > Environmental and Occupational Health Sciences > 4225 Roosevelt Way NE, # 100 > Seattle WA 98105-6099 > [[alternative HTML version deleted]] > > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
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I am planning to use read.table to create the objects that feed to GOFrame. The manual says that the three columns are required, GO IDs, evidence code and gene IDs. I think I understand the first and third column, but what information should I put in evidence code? For example I have the following in text file: --------------------------------- GO:0002576    ***    gene1 ------------------------------- Does it work? [[alternative HTML version deleted]]
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Just realized some typo in the subject of previous post. So would like to raise this question again since nobody answer it last time. This is relevant to usage of GOstat package with unsupported model organism. Thanks. ________________________________ To: "bioconductor@r-project.org" <bioconductor@r-project.org> Sent: Wednesday, January 16, 2013 9:38 AM Subject: question about "evidence cole" in data.frame from GOstats packages with unsupported model organisms I am planning to use read.table to create the objects that feed to GOFrame. The manual says that the three columns are required, GO IDs, evidence code and gene IDs. I think I understand the first and third column, but what information should I put in evidence code? For example I have the following in text file: --------------------------------- GO:0002576    ***    gene1 ------------------------------- Does it work? [[alternative HTML version deleted]]
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Hi, The evidence codes are defined by the folks who work on GO, and it refers to the kind of evidence that supports applying a particular GO term to a specific gene ID. For many genes to GO mappings in non- model organisms the association is "second hand", meaning that it was really studied on another organism and then assumed to also apply in this other organism (often because of sequence homology). Here is a web site that lists some acceptable evidence codes: http://www.geneontology.org/GO.evidence.shtml Hope this helps, Marc On 01/21/2013 09:07 AM, Fix Ace wrote: > Just realized some typo in the subject of previous post. So would like to raise this question again since nobody answer it last time. This is relevant to usage of GOstat package with unsupported model organism. > > Thanks. > > > ________________________________ > > To: "bioconductor@r-project.org"<bioconductor@r-project.org> > Sent: Wednesday, January 16, 2013 9:38 AM > Subject: question about "evidence cole" in data.frame from GOstats packages with unsupported model organisms > > > I am planning to use read.table to create the objects that feed to GOFrame. The manual says that the three columns are required, GO IDs, evidence code and gene IDs. I think I understand the first and third column, but what information should I put in evidence code? > > For example I have the following in text file: > --------------------------------- > > GO:0002576 *** gene1 > > ------------------------------- > > Does it work? > [[alternative HTML version deleted]] > > > > _______________________________________________ > Bioconductor mailing list > Bioconductor@r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor [[alternative HTML version deleted]]
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