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Ian Sudbery
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30
@ian-sudbery-5757
Last seen 10.2 years ago
Hi All,
I was wondering if anyone had any idea how one might
put error bars on the log fold changes calculated by edgeR? I'd like
to do this so I can show examples of differentially expressed genes on
a bar plot, and show them in comparison to qPCR validation.
I feel like one should be able to get the variance from the
dispersion:
CV^2 = 1/mu + BCV^2 = 1/mu + dispersion
So:
variance = mu + mu^2*dispersion ?
This could either be used to show the variance for each of the two
conditions, or used to calculate an error for the fold change. but I'm
a bit stuck as to how to actually implement this.
Any ideas appreciated,
Yours,
Ian
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