Finding closest gene to chromosomal position (non-human or mouse)
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Molly Hanlon ▴ 20
@molly-hanlon-5792
Last seen 10.2 years ago
Hi list, I've been using R for some time, but am new to bioconductor/biomaRt. Please pardon that. I have a list of a couple thousand SNPs and I'm looking for the closest gene to each of them. Looking just within the gene, I only get a result for about a third of them, so I obviously need something a bit more robust. Searching the archives, I've found mention of the findClosestGene in ACME, however, I work in rice, so this isn't much help. Ideally, I'd like to enter a position, say "9:12344567" and get an output that it lies 1234 bp upstream from Loc_OsXXXXXX. I've also seen suggestions that include getting a data frame of all the gene positions and then using findOverlap with my frame, though I'm not sure this would work because I have no strand information for my query and they're single base pairs, thus without a range. Feel free to correct my assumptions, but any additional help you can provide would be wonderful. Many thanks, Molly [[alternative HTML version deleted]]
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Tim Triche ★ 4.2k
@tim-triche-3561
Last seen 4.2 years ago
United States
if there is a TranscriptDb for rice, the VariantAnnotation package will be super handy. Given a GenomicRanges with (chromosome, start, end) for each variant (not necessarily a SNP, could be an indel or repeat region!), the package will find the nearest gene/transcript, the type of predicted consequence, and the relationship for each variant... IFF there is a TranscriptDb or GRanges representation of where these genes are. If there is not a TranscriptDb for rice, you might want to create one... VariantAnnotation is *that useful*. Just a thought! Best, --t On Mon, Feb 25, 2013 at 1:35 PM, Molly Hanlon <hanlonmt@gmail.com> wrote: > Hi list, > > I've been using R for some time, but am new to bioconductor/biomaRt. Please > pardon that. > > I have a list of a couple thousand SNPs and I'm looking for the closest > gene to each of them. Looking just within the gene, I only get a result > for about a third of them, so I obviously need something a bit more robust. > Searching the archives, I've found mention of the findClosestGene in ACME, > however, I work in rice, so this isn't much help. Ideally, I'd like to > enter a position, say "9:12344567" and get an output that it lies 1234 bp > upstream from Loc_OsXXXXXX. > > I've also seen suggestions that include getting a data frame of all the > gene positions and then using findOverlap with my frame, though I'm not > sure this would work because I have no strand information for my query and > they're single base pairs, thus without a range. > > Feel free to correct my assumptions, but any additional help you can > provide would be wonderful. > > Many thanks, > Molly > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor@r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > -- *A model is a lie that helps you see the truth.* * * Howard Skipper<http: cancerres.aacrjournals.org="" content="" 31="" 9="" 1173.full.pdf=""> [[alternative HTML version deleted]]
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Julie Zhu ★ 4.3k
@julie-zhu-3596
Last seen 12 months ago
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Molly, You might want to try annotatePeakInBatch in ChIPpeakAnno package with parameter output="both". Best regards, Julie On 2/25/13 4:35 PM, "Molly Hanlon" <hanlonmt at="" gmail.com=""> wrote: > Hi list, > > I've been using R for some time, but am new to bioconductor/biomaRt. Please > pardon that. > > I have a list of a couple thousand SNPs and I'm looking for the closest > gene to each of them. Looking just within the gene, I only get a result > for about a third of them, so I obviously need something a bit more robust. > Searching the archives, I've found mention of the findClosestGene in ACME, > however, I work in rice, so this isn't much help. Ideally, I'd like to > enter a position, say "9:12344567" and get an output that it lies 1234 bp > upstream from Loc_OsXXXXXX. > > I've also seen suggestions that include getting a data frame of all the > gene positions and then using findOverlap with my frame, though I'm not > sure this would work because I have no strand information for my query and > they're single base pairs, thus without a range. > > Feel free to correct my assumptions, but any additional help you can > provide would be wonderful. > > Many thanks, > Molly > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor
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I asked a similar question last week and got the solution with the help of Valerie. I was looking for flanking genes and not necessarily the closest. If you are interested, see my blog at http://adairama.wordpress.com/2013/02/15/functionally-annotate-snps- and-indels-in-bioconductor/ Regards, Adai On Tue, Feb 26, 2013 at 1:21 PM, Zhu, Lihua (Julie) <julie.zhu@umassmed.edu>wrote: > Molly, > > You might want to try annotatePeakInBatch in ChIPpeakAnno package with > parameter output="both". > > Best regards, > > Julie > > > On 2/25/13 4:35 PM, "Molly Hanlon" <hanlonmt@gmail.com> wrote: > > > Hi list, > > > > I've been using R for some time, but am new to bioconductor/biomaRt. > Please > > pardon that. > > > > I have a list of a couple thousand SNPs and I'm looking for the closest > > gene to each of them. Looking just within the gene, I only get a result > > for about a third of them, so I obviously need something a bit more > robust. > > Searching the archives, I've found mention of the findClosestGene in > ACME, > > however, I work in rice, so this isn't much help. Ideally, I'd like to > > enter a position, say "9:12344567" and get an output that it lies 1234 bp > > upstream from Loc_OsXXXXXX. > > > > I've also seen suggestions that include getting a data frame of all the > > gene positions and then using findOverlap with my frame, though I'm not > > sure this would work because I have no strand information for my query > and > > they're single base pairs, thus without a range. > > > > Feel free to correct my assumptions, but any additional help you can > > provide would be wonderful. > > > > Many thanks, > > Molly > > > > [[alternative HTML version deleted]] > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor@r-project.org > > https://stat.ethz.ch/mailman/listinfo/bioconductor > > Search the archives: > > http://news.gmane.org/gmane.science.biology.informatics.conductor > > _______________________________________________ > Bioconductor mailing list > Bioconductor@r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > [[alternative HTML version deleted]]
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Great! I was able to use annotatePeakInBatch as per Julie's recommendation to get what I was looking for. Thanks for the help! On Tue, Feb 26, 2013 at 9:10 AM, Adaikalavan Ramasamy < adaikalavan.ramasamy@gmail.com> wrote: > I asked a similar question last week and got the solution with the help of > Valerie. I was looking for flanking genes and not necessarily the closest. > If you are interested, see my blog at > http://adairama.wordpress.com/2013/02/15/functionally-annotate-snps- and-indels-in-bioconductor/ > > Regards, Adai > > > On Tue, Feb 26, 2013 at 1:21 PM, Zhu, Lihua (Julie) < > Julie.Zhu@umassmed.edu> wrote: > >> Molly, >> >> You might want to try annotatePeakInBatch in ChIPpeakAnno package with >> parameter output="both". >> >> Best regards, >> >> Julie >> >> >> On 2/25/13 4:35 PM, "Molly Hanlon" <hanlonmt@gmail.com> wrote: >> >> > Hi list, >> > >> > I've been using R for some time, but am new to bioconductor/biomaRt. >> Please >> > pardon that. >> > >> > I have a list of a couple thousand SNPs and I'm looking for the closest >> > gene to each of them. Looking just within the gene, I only get a result >> > for about a third of them, so I obviously need something a bit more >> robust. >> > Searching the archives, I've found mention of the findClosestGene in >> ACME, >> > however, I work in rice, so this isn't much help. Ideally, I'd like to >> > enter a position, say "9:12344567" and get an output that it lies 1234 >> bp >> > upstream from Loc_OsXXXXXX. >> > >> > I've also seen suggestions that include getting a data frame of all the >> > gene positions and then using findOverlap with my frame, though I'm not >> > sure this would work because I have no strand information for my query >> and >> > they're single base pairs, thus without a range. >> > >> > Feel free to correct my assumptions, but any additional help you can >> > provide would be wonderful. >> > >> > Many thanks, >> > Molly >> > >> > [[alternative HTML version deleted]] >> > >> > _______________________________________________ >> > Bioconductor mailing list >> > Bioconductor@r-project.org >> > https://stat.ethz.ch/mailman/listinfo/bioconductor >> > Search the archives: >> > http://news.gmane.org/gmane.science.biology.informatics.conductor >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor@r-project.org >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor >> > > [[alternative HTML version deleted]]
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